The objective of the study was to assess the integrated use of macroinvertebrate indexes and ecotoxicological parameters in the evaluation of the ecological status of a temporary stream with a strong agricultural influence. Water quality was analysed at two sampling sites along the stream, considering: chemical supporting parameters; hazardous substances (pesticides); benthic macroinvertebrate communities, through quality (Iberian Biological Monitoring Working Party and Iberian Average Score Per Taxon) and multi-metric indices (Southern Portuguese Index of Invertebrates and Ecological Quality Ratio); and ecotoxicological responses using lethal and sub-lethal bioassays. The water chemical characterization showed high levels of organic matter and nutrients, mainly in the dry period ((biochemical oxygen demand (BOD₅): 18.5–25.5 mg L⁻¹, chemical oxygen demand (COD): 60.8–193.7 mg L⁻¹; total phosphorus (TP): 0.17–0.33 mg L⁻¹)), which may compromise the support of biological life. In accordance with the physicochemical results, the stream had an ecological status less than good. Of the 25 pesticides analysed, only five, namely terbuthylazine, 2-methyl-chlorophenoxyacetic acid, bentazone, mecoprop and metolachlor were quantified. In general, the concentrations of pesticides detected were low, except at the source of the stream in January 2012 (sum of pesticides 2.29 μg L⁻¹), mainly due to the concentration of bentazone (1.77 μg L⁻¹), both values surpassing the European Commission threshold values. The analysis of benthic macroinvertebrates showed low levels of abundance and family diversity, with communities dominated by resistant groups to organic pollution and pesticides, such as the Chironomidae family. In general, the reproduction ecotoxicological results showed a very marked decrease in the number of juveniles per female. The Spearman correlation identified pesticides, namely MCPA (R = −0.89; p < 0.05), as the main responsible for the observed effect. The results showed the linearity and complementarity of the two groups of biological responses, allowing to cover the interactions between the ecosystem's species and the different types of pollutants.

这项研究的目的是评估大无脊椎动物指数和生态毒理学参数在评估具有强烈农业影响的临时河流的生态状况时的综合利用。在沿河的两个采样点对水质进行了分析，考虑到：化学支持参数；有害物质（农药）；通过质量（伊比利亚生物监测工作组和伊比利亚每分类群平均得分）和多指标指数（南葡萄牙无脊椎动物和生态质量比）的底栖大型无脊椎动物群落；以及使用致死和亚致死生物测定的生态毒理反应。水化学特征显示出高水平的有机质和养分，主要是在干旱时期（（生化需氧量（BOD ₅）：18.5–25.5 mg L ^-1，化学需氧量（COD）：60.8–193.7 mg L ^-1；总磷（TP）：0.17–0.33 mg L ^-1）），这可能会损害生物生命的支持。根据理化结果，该溪流的生态状况不佳。在分析的25种农药中，仅对5种化合物进行了定量，即丁苯噻嗪，2-甲基-氯苯氧基乙酸，苯达松，甲丙酸和异丙甲草胺。通常，农药的浓度检测到的低，除了在流的源在2012年1月（农药的总和2.29微克大号^-1），这主要是由于苯达松（1.77微克L的浓度^-1），这两个值均超过了欧盟委员会的阈值。对底栖大型无脊椎动物的分析显示，其丰度和家庭多样性水平较低，其社区以对有机污染和农药的抗药性群体为主导，例如Chironomidae家族。一般而言，生殖生态毒理学结果表明，每只雌性幼鱼的数量大大减少。Spearman相关性确定了农药，即MCPA（R  = −0.89；p  <0.05），是造成观察到的效果的主要原因。结果表明，两组生物响应具有线性和互补性，从而涵盖了生态系统物种与不同类型污染物之间的相互作用。