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Authentication of edible fats and oils by non-targeted 13C INEPT NMR spectroscopy
Food Control ( IF 6 ) Pub Date : 2018-04-07
Sophie Guyader, Freddy Thomas, Vincent Portaluri, Eric Jamin, Serge Akoka, Virginie Silvestre, Gérald Remaud

Further to recent food scandals, consumers have become increasingly concerned about the quality and integrity of the processed products they buy and eat. Edible oils contaminations or deliberate adulterations are recurring issues. Until now the lipidic composition of food has not been extensively studied by Nuclear Magnetic Resonance (NMR) spectroscopy despite the fact that this analytical method can cover a wide range of matrices. The application of a refocused adiabatic 13C INEPT (Insensitive Nuclei Enhanced by Polarization Transfer) sequence has already proved the performance of NMR spectroscopy to discriminate the origins of olive oils. This sequence has been demonstrated to be faster than a classical 13C NMR sequence. In this work, the implementation of that 13C NMR sequence in a routine laboratory has allowed the analysis of 23 common origins of edible oils, as well as butters, margarines and animal fats. We have proven that butter origin could clearly be distinguished from plant oil origins considered in this work. With the example of the verification of palm oil presence in food products, we have demonstrated the applicability of the method to characterize fats origin in transformed products. This study has shown by means of chemometric tools the potential of 13C NMR spectroscopy to check the authenticity of various commercial finished products, using an untargeted approach applied to a spectral database of 294 spectra of raw animal and plant fats and oils. Thanks to spiking tests, the limit of detection of one origin of plant oil in another has been estimated as being around 5% and the limit of detection of animal oil in plant oils has been estimated as being around 2%. The same approach can be applied as a global method to verify the authenticity and integrity of fats and oils.



中文翻译:

通过非靶向13 C INEPT NMR光谱鉴定食用油脂

除最近的食品丑闻外,消费者越来越关注他们购买和食用的加工产品的质量和完整性。食用油污染或故意掺假是经常出现的问题。迄今为止,尽管该分析方法可以覆盖多种基质,但尚未通过核磁共振(NMR)光谱法对食物的脂质成分进行广泛研究。重新聚焦的绝热13 C INEPT(通过极化转移增强的不灵敏核)序列的应用已经证明了NMR光谱学能够分辨橄榄油的来源。已证明该序列比经典的13 C NMR序列更快。在这项工作中,执行该13常规实验室中的13 C NMR序列分析了23种常见的食用油以及黄油,人造黄油和动物脂肪。我们已经证明,黄油的来源可以与本工作中考虑的植物油明显区别开来。以验证食品中棕榈油的存在为例,我们证明了该方法可用于表征转化产品中脂肪的来源。这项研究通过化学计量学工具显示了13种潜在的1 C NMR光谱学检查各种商业成品的真实性,使用了一种无针对性的方法,该方法应用于294种动植物油脂的光谱数据库。由于进行了尖峰测试,一种植物油中另一种植物的检出限估计为5%左右,而植物油中动物油的检出限估计为2%左右。可以将同一方法用作验证油脂的真实性和完整性的全局方法。

更新日期:2018-04-08
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