Herein, the ability of ginger glycoproteins to inhibit the angiotensin-converting enzyme (ACE) is characterized. The activity is monitored via HPLC, and then the crude glycoproteins are enriched with lectin microarrays and magnetic microspheres. The N-linked glycans released from the enriched glycoproteins by PNGase F are identified by MALDI-TOF-MS. The results suggest that the crude ginger glycoproteins are active against ACE with an IC₅₀ value of 0.83 ± 0.09 mg mL⁻¹. The ginger glycoproteins are enriched by concanavalin A (Con A) and solanum tuberosum (Potato) lectin (STL), and the structures of the N-glycans released from the ginger glycoproteins include high-mannose type glycans, fucosylated-type glycans, and hybrid-type glycans, as analyzed by MALDI-TOF-MS. The results of this study are expected to provide a reference for the glycan structure of ginger glycoproteins with ACE-inhibitory activity.

中文翻译：

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具有ACE抑制活性的姜中N-连接糖蛋白的MALDI-TOF-MS表征

在本文中，表征了姜糖蛋白抑制血管紧张素转化酶（ACE）的能力。通过HPLC监测活性，然后用凝集素微阵列和磁性微球富集粗糖蛋白。通过MALDI-TOF-MS鉴定通过PNGase F从富集的糖蛋白释放的N-连接的聚糖。结果表明，粗姜糖蛋白具有抗ACE活性，IC ₅₀值为0.83±0.09 mg mL ^-1。姜糖蛋白富含伴刀豆球蛋白A（Con A）和马铃薯马铃薯（马铃薯）凝集素（STL），以及N的结构。通过MALDI-TOF-MS分析，从姜糖蛋白释放的β-聚糖包括高甘露糖型聚糖，岩藻糖基化型聚糖和杂合型聚糖。该研究结果有望为具有ACE抑制活性的姜糖蛋白的聚糖结构提供参考。