The detection and inhibition of DNA damage are of great importance in the prevention and treatment of diseases. Developing a simple and sensitive tool for this purpose would be a chance to monitor the DNA damage and could be helpful in introducing some drugs which can prevent this phenomenon. Here, we report a novel and sensitive electrochemical biosensor based on DNA/Au nanoparticles (AuNPs) modified screen printed gold electrode (DNA/AuNPs/SPGE) to investigate the DNA damage process and also to study the protective behavior of deferoxamine (DFO). The proposed biosensor was fabricated by electrodeposition of AuNPs onto SPGE, followed by chemical immobilisation of thiol-terminated DNA. Cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and scanning electron microscopy (SEM) have been used to characterise this biosensor. Hydroxyl radical (OH), which is generated during the Fenton reaction, is responsible for the induced damage to DNA. EIS technique was applied to monitor the DNA damage, and the increase in charge transfer resistance (R_ct) following the DNA damage, was considered as an indicator. Furthermore, the ability of the electrochemical screening system was proved by the investigation of the antioxidant effect of DFO in prohibiting the DNA damage.

DNA损伤的检测和抑制在疾病的预防和治疗中非常重要。为此目的开发一种简单而灵敏的工具将有机会监测DNA损伤，并有助于引入一些可以预防这种现象的药物。在这里，我们报告基于DNA / Au纳米粒子（AuNPs）修饰的丝网印刷金电极（DNA / AuNPs / SPGE）的新型且灵敏的电化学生物传感器，以研究DNA损伤过程并研究去铁胺（DFO）的保护行为。拟议的生物传感器是通过将AuNPs电沉积到SPGE上，然后化学固定巯基封端的DNA制成的。循环伏安法（CV），电化学阻抗谱（EIS）和扫描电子显微镜（SEM）已用于表征该生物传感器。在Fenton反应过程中产生的OH）导致了对DNA的诱导破坏。EIS技术用于监测DNA损伤，并且DNA损伤后电荷转移阻力（R _ct）的增加被视为指示剂。此外，通过研究DFO在防止DNA损伤方面的抗氧化作用证明了电化学筛选系统的能力。