We have comprehensively investigated the structure-stability relationship of natural polyphenols in DMEM medium without cells. Polyphenols with catechol or pyrogallol structure were evidently instable in DMEM medium without cells. Herein, we further investigate stability of polyphenols when incubated with cancer cells and its related mechanism. After incubated with SK-28 cells and A549 cells at 37 °C in 5% CO₂ for 72 h, the new products of quercetin and 5,7,3′,4′-tetrahydroxyflavone were found to quite different from different cells. It is time to investigate what really happened for polyphenols and the new products of polyphenols in cancer cells, as well as the related mechanism. It is very important to further check the bioactivity of these new products, which will avoid erroneous conclusions for what's the really bioactive compounds.

我们已经全面研究了天然多酚在无细胞的DMEM培养基中的结构稳定性关系。在没有细胞的DMEM培养基中，具有邻苯二酚或邻苯三酚结构的多酚显然不稳定。在本文中，我们进一步研究了多酚与癌细胞孵育时的稳定性及其相关机制。与SK-28细胞和A549细胞在5％CO _2中于37°C孵育后在72小时内，发现槲皮素和5,7,3'，4'-四羟基黄酮的新产物与不同细胞完全不同。现在是时候研究癌细胞中多酚和多酚新产品的实际情况，以及相关的机制。进一步检查这些新产品的生物活性非常重要，这样可以避免对真正的生物活性化合物有错误的结论。