One of the most challenging problems when trying to recycle urine for different purposes is the removal of urea. In this project we studied an ureolysis system using the bacterium Proteus vulgaris for the transformation of urea to ammonia and its subsequent oxidation to nitrogen at a Pt working electrode. Our system was tested under different pH, microbial reaction times, and urea and bacteria concentrations. Our results indicate that a pH 8 is optimal for the combined Proteus vulgaris urease activity and the ammonia oxidation reaction at a Pt electrode. The reaction time and concentration dependence on the ammonia oxidation reaction current densities was also studied. Results showed limited ammonia oxidation under high urea concentrations in ~2.5 × 10⁹ cfu/mL Proteus vulgaris in synthetic urine.

尝试为不同目的回收尿液时最具挑战性的问题之一是尿素的去除。在这个项目中，我们研究了一种利用普通变形杆菌细菌将尿素转化为氨并随后在Pt工作电极上氧化为氮的尿素分解系统。我们的系统在不同的pH值，微生物反应时间，尿素和细菌浓度下进行了测试。我们的结果表明，pH 8最适合组合的寻常变形杆菌脲酶活性和Pt电极上的氨氧化反应。还研究了反应时间和浓度对氨氧化反应电流密度的依赖性。结果表明，在约2.5×10 ^9的高尿素浓度下，氨氧化受到限制 cfu / mL合成尿中的变形杆菌。