Modified reinforced clostridial medium (mRCM) was developed and evaluated for the differential enumeration of Lactobacillus delbrueckii ssp. bulgaricus. Lactobacillus bulgaricus, an important species of lactic acid bacteria with health benefits, is used in the production of yogurt and other fermented foods. Our results showed that supplementing reinforced clostridial medium with 0.025% CaCl₂, 0.01% uracil, and 0.2% Tween 80 (mRCM) significantly enhanced the growth rate of L. bulgaricus RR and ATCC 11842 strains as measured by the optical densities of these strains after 12 h of incubation at 42°C. The bacterial populations (plate count) of the RR and ATCC 11842 strains were 0.76 and 0.77 log cfu/g higher in mRCM than in de Man, Rogosa, and Sharpe and reinforced clostridial medium media, respectively. Conversely, the population counts for other bacterial species (Bifidobacterium, Lactobacillus rhamnosus, and Lactobacillus reuteri) were significantly inhibited in the mRCM medium. The addition of aniline blue dye to mRCM (mRCM-blue) improved the selectivity of L. bulgaricus in mixed lactic bacterial cultures compared with de Man, Rogosa, and Sharpe medium and lactic agar with regard to colony appearance and morphology. The mRCM-blue performed better than the conventional medium in culturing, enumerating, and differentiating L. bulgaricus. Therefore, mRCM-blue could be used as a selective medium to enhance the growth and differentiation of L. bulgaricus in order to meet the increasing demand for this beneficial species of bacteria.

开发了改良的增强型梭菌培养基（mRCM）并评估了德氏乳杆菌属（Lactobacillus delbrueckii ssp）的差异计数。保加利亚。保加利亚乳杆菌是一种对人体有益的重要乳酸菌，可用于酸奶和其他发酵食品的生产。我们的研究结果表明，补充强化梭菌培养基用0.025％的CaCl ₂，0.01％尿嘧啶，和0.2％吐温80（MRCM）显著增强的生长速率保加利亚乳杆菌RR和ATCC 11842菌株，通过在42°C孵育12小时后通过这些菌株的光密度测量。RR和ATCC 11842菌株的细菌种群（平板数）在mRCM中分别比在De Man，Rogosa和Sharpe和增强型梭菌培养基中高0.76和0.77 log cfu / g。相反，在mRCM培养基中，其他细菌种类（双歧杆菌，鼠李糖乳杆菌和罗伊氏乳杆菌）的种群计数被显着抑制。在mRCM（mRCM-blue）中添加苯胺蓝染料可提高保加利亚乳杆菌的选择性就菌落外观和形态而言，与德曼，罗戈萨和夏普培养基和乳酸琼脂相比，混合乳酸菌培养液中的细菌含量要高。在培养，枚举和区分保加利亚乳杆菌方面，mRCM-blue的性能优于传统培养基。因此，mRCM-blue可以用作选择性培养基，以增强保加利亚乳杆菌的生长和分化，从而满足对该有益细菌物种不断增长的需求。