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Jugular arginine infusion relieves lipopolysaccharide-triggered inflammatory stress and improves immunity status of lactating dairy cows
Journal of Dairy Science ( IF 3.5 ) Pub Date : 2018-04-05
F.F. Zhao, T.Y. Wu, H.R. Wang, L.Y. Ding, Gulzar Ahmed, H.W. Li, W. Tian, Y.Z. Shen

The objective of this study was to evaluate the effects of jugular l-Arg infusion on performance and immune function during lipopolysaccharide (LPS)-induced inflammation of lactating dairy cows. Eight Holstein cows (multiparous, 608.8 ± 31.5 kg) at mid-lactation were randomly assigned to 5-d jugular infusions of control (saline), Arg (3 g/h), LPS (0.033 μg/kg per h), and LPS + Arg (0.033 μg/kg per h of LPS and 3 g/h of Arg) in a replicated 4 × 4 Latin square design with 4 infusion periods separated by 10-d noninfusion periods. Jugular solutions of saline, Arg, LPS, and LPS + Arg were continuously infused using peristaltic pumps for approximately 6 h/d during infusion periods. Milk yield was measured on each day of the infusion period. Milk samples were obtained on the last 2 d of each infusion period, and blood samples were obtained on the last day of each infusion period before infusion (0 h) and at 3 and 6 h. We found that the jugular LPS infusion significantly increased serum concentrations of IL-1β, IL-6, tumor necrosis factor, inducible nitric oxide synthase, and lipopolysaccharide binding protein, whereas Arg attenuated the increase in IL-6 and inducible nitric oxide synthase levels and tended to decrease the lipopolysaccharide binding protein level. Arginine alleviated the decrease in dry matter intake and milk fat yield and the increase of somatic cell count induced by LPS. Total casein in milk was decreased during the LPS-induced inflammation period, and jugular Arg infusion significantly increased the content of total casein. In contrast, lactalbumin in milk increased during the LPS-induced inflammation period, whereas jugular Arg infusion significantly decreased the content of lactalbumin. The concentrations of plasma Gly, Thr, Ile, Leu, Arg, Phe, and total free AA were significantly decreased by LPS treatment, but Arg attenuated this tendency. These results indicated that jugular Arg infusion (18 g/d) has protective effects on relieving inflammatory stress and improving immunity status triggered by LPS. In conclusion, Arg could attenuate inflammatory stress and improve milk performance of lactating dairy cows. This protective effect may be due to the ability of Arg to suppress LPS effects and improve immunity status.



中文翻译:

颈精氨酸输注可缓解脂多糖触发的炎症应激,并改善泌乳奶牛的免疫状况

这项研究的目的是评估颈的作用脂多糖(LPS)诱导的泌乳奶牛炎症过程中-Arg输注对性能和免疫功能的影响。在泌乳中期,将八头荷斯坦奶牛(多胎,608.8±31.5公斤)随机分配至5天颈静脉输注对照(盐水),精氨酸(3克/小时),脂多糖(0.033微克/公斤/小时)和脂多糖+精氨酸(0.033μg/ kg / h LPS和3 g / h精氨酸),采用重复的4×4拉丁方形设计,其中4个输液周期被10天非输液周期分隔。在输液期间,使用蠕动泵连续输注盐水,Arg,LPS和LPS + Arg的颈静脉溶液约6 h / d。在输液期间的每一天测量牛奶产量。在每个输液期的最后2天获取牛奶样本,在每个输液期的最后一天在输液之前(0小时)以及3和6小时获取血液样本。我们发现颈静脉LPS输注显着增加了血清IL-1β,IL-6,肿瘤坏死因子,诱导型一氧化氮合酶和脂多糖结合蛋白的浓度,而Arg减弱了IL-6和诱导型一氧化氮合酶水平的升高,以及倾向于降低脂多糖结合蛋白水平。精氨酸减轻了LPS引起的干物质摄入量和乳脂产量的减少以及体细胞计数的增加。在LPS诱发的炎症期间,牛奶中的总酪蛋白含量降低,而颈动脉Arg输注显着增加了总酪蛋白含量。相反,在LPS诱导的炎症期间,牛奶中的乳白蛋白增加,而颈动脉Arg输注显着降低乳白蛋白的含量。血浆Gly,Thr,Ile,Leu,Arg的浓度 LPS处理显着降低了Phe和总游离AA,但Arg减弱了这种趋势。这些结果表明,颈动脉精氨酸输注(18 g / d)对减轻炎症应激和改善LPS触发的免疫状态具有保护作用。总之,精氨酸可以减轻乳牛的炎性应激并改善其泌乳性能。这种保护作用可能归因于Arg抑制LPS作用和改善免疫状态的能力。

更新日期:2018-04-06
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