A new-type fluorescent probe HPQ-βGal based on aggregation induced emission (AIE) and excited state intramolecular proton transfer (ESIPT) is designed, showing a superior detection performance to β-galactosidase activity. HPQ-βGal regulates the β-galactopyranoside to react with β-gal and release the chromophore. The probe proves favorable advantages, such as high sensitivity and selectivity, convenient handling procedures, a large Stokes shift and self-exciting at an aggregated condition. Moreover, HPQ-βGal was also applied in detecting of endogenous and exogenous β-galactosidase activity in OVCAR-3 cells, and the imaging was achieved with high contrast benefitted from its good retention in living cells.

设计了一种基于聚集诱导发射（AIE）和激发态分子内质子转移（ESIPT）的新型荧光探针HPQ-βGal，具有优于β-半乳糖苷酶活性的检测性能。HPQ-βGal调节β-吡喃半乳糖苷与β-gal反应并释放发色团。该探头具有良好的优势，例如灵敏度高，选择性好，操作程序方便，斯托克斯位移大以及在聚集条件下自激。此外，HPQ-βGal还用于检测OVCAR-3细胞的内源性和外源性β-半乳糖苷酶活性，得益于其在活细胞中的良好保留，可实现高对比度成像。