This study aimed to establish a mathematical modeling to evaluate the inhibitory effect of phenolic derivatives on acetone-butanol-ethanol (ABE) fermentation by Clostridium saccharoperbutylacetonicum N1-4. Vanillin, 4-hydroxybenzoic acid, and syringaldehyde were selected to represent guaiacyl, hydroxyphenyl, and syringyl phenols, respectively, to be examined in a series of fed-batch experiments. Results show the presence of phenolic derivatives blocked the pathway of the assimilation of organic acids and reduced cell growth and glucose utilization. The inhibition model projected that the levels of 0.13, 0.14, and 0.04 g L^-1 for vanillin, 4-hydroxybenzoic acid, and syringaldehyde, respectively, resulted in 25% inhibition of butanol production, whereas 100% inhibition was predicted at the levels of 4.94, 4.37, and 4.20 g L^-1 for vanillin, 4-hydroxybenzoic acid, and syringaldehyde, respectively. Syringaldehyde was more toxic than the other two compounds. The established model described that the phenolic compounds derived from different phenyl propane monomers of lignin severely obstructed biobutanol production.

这项研究旨在建立数学模型，以评估酚类衍生物对蔗糖丁醇梭状芽孢杆菌N1-4丙酮-丁醇-乙醇（ABE）发酵的抑制作用。选择香兰素，4-羟基苯甲酸和丁香醛分别代表愈创木酚，羟苯基和丁香烯基苯酚，将在一系列补料分批实验中进行检查。结果表明，酚类衍生物的存在阻止了有机酸同化的途径，并减少了细胞的生长和葡萄糖的利用。抑制模型预测0.13、0.14和0.04 g L ^-1的水平分别对香兰素，4-羟基苯甲酸和丁香醛产生25％的丁醇抑制作用，而对于香兰素，4-羟基苯甲酸，则分别以4.94、4.37和4.20 g L ^-1的水平预测100％抑制作用，和丁香醛。丁二醛的毒性比其他两种化合物高。建立的模型描述了衍生自木质素的不同苯基丙烷单体的酚类化合物严重阻碍了生物丁醇的生产。