An accurate and precise 3 μL blood collection and dispensing system is presented for the preparation of dried blood spot (DBS) samples. Using end-to-end glass capillaries in conjugation with pre-punched DBS pads, a blood micro collection system was developed to eliminate the haematocrit dispersion, widely associated with DBS technology, while providing better levels of accuracy and precision during sample preparation. This methodology is compared to traditional micro-volume blood collection systems, such as a pipette and a digitally controlled analytical syringe. Results showed that % of recovery for the capillary methodology was closer to 100% across the three haematocrit (HCT) levels tested and when prepared by two users (98 to 100% for capillaries, 78 to 104% for pipette and 93 to 97% for digital syringe) attesting a higher accuracy. Additionally, by taking advantage of the capillary action mechanism to collect and dispense autonomously the desired volume of blood onto the DBS pad, coefficients of variation between two individuals were significantly lower than with standard methodologies (capillaries—0.05 to 0.77%, pipette—12.71 to 18.53% and digital syringe—0.72 to 1.77%). This alternate aspiration and dispensing methodology could be used by different users without compromising accuracy or precision when handling low volumes of blood during the pre-analytical steps.

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提出了一种精确，精确的3μL血液采集和分配系统，用于制备干血斑（DBS）样品。通过将端到端玻璃毛细管与预先打孔的DBS垫结合使用，开发了血液微收集系统，以消除与DBS技术广泛相关的血细胞比容分散，同时在样品制备过程中提供更高水平的准确性和精密度。将该方法与传统的微量采血系统（例如移液器和数控分析注射器）进行了比较。结果表明，在两个用户使用的三种血细胞比容（HCT）水平下，毛细血管方法的回收率接近100％（两个毛细管（毛细管的98％至100％，移液器的78％至104％，毛细管的93％至97％））数字注射器）证明了更高的准确性。此外，通过利用毛细管作用机制自动将所需量的血液收集和分配到DBS垫上，两个人之间的变异系数显着低于标准方法（毛细管-0.05至0.77％，移液器-12.71至18.53％和数字注射器-0.72至1.77％）。在分析前步骤中处理少量血液时，不同的使用者可以使用这种交替的抽吸和分配方法，而不会影响准确性或精密度。53％和数字注射器-0.72至1.77％）。在分析前步骤中处理少量血液时，不同的使用者可以使用这种交替的抽吸和分配方法，而不会影响准确性或精密度。53％和数字注射器-0.72至1.77％）。在分析前步骤中处理少量血液时，不同的使用者可以使用这种交替的抽吸和分配方法，而不会影响准确性或精密度。

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