A new chemosensor based on coumarin FB has been designed and synthesized for the detection of Fe³⁺ and PPi. FB displayed a high affinity to Fe³⁺ in the presence of other competing cations. The resulting FB-Fe³⁺ complex displayed highly sensitivity to PPi via Fe³⁺ displacement approach. The Fe³⁺ and PPi recognition processes is rapid and reversible, the detection limits of FB to Fe³⁺ and FB-Fe³⁺ complex to PPi were estimated to be 8.73 × 10⁻⁸ M and 1.25 × 10⁻⁸ M, respectively. The good biocompatibility of FB enables the investigation of fluorescent response for Fe³⁺ and PPi in living cells by confocal microscope. A B3LYP/6-31G(d,p) basis set was employed for optimization of FB and FB-Fe³⁺ complex.

设计并合成了一种基于香豆素FB的新型化学传感器，用于检测Fe ³⁺和PPi。在其他竞争性阳离子的存在下，FB对Fe ³⁺具有很高的亲和力。所得的FB -Fe ³⁺复合物通过Fe ³⁺置换方法对PPi表现出高度的敏感性。Fe ³⁺和PPi的识别过程快速且可逆，据估计，FB对Fe ³⁺的检出限和FB -Fe ³⁺对PPi的检出限估计为8.73×10 ^-8  M和1.25×10 ^-8 M分别。FB具有良好的生物相容性，可通过共聚焦显微镜研究活细胞中Fe ³⁺和PPi的荧光响应。B3LYP / 6-31G（d，p）基础集用于优化FB和FB -Fe ^3+配合物。