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UHPLC-QqQ-MS/MS identification, quantification of polyphenols from Passiflora subpeltata fruit pulp and determination of nutritional, antioxidant, α-amylase and α-glucosidase key enzymes inhibition properties
Food Research International ( IF 8.1 ) Pub Date : 2018-04-04 , DOI: 10.1016/j.foodres.2018.04.006
Saravanan Shanmugam , Isla Alcântara Gomes , Marina Denadai , Bruno dos Santos Lima , Adriano Antunes de Souza Araújo , Narendra Narain , Maria Terezinha Santos Leite Neta , Mairim Russo Serafini , Lucindo José Quintans-Júnior , Parimelazhagan Thangaraj

The diabetic key enzymes inhibition, nutritional, antioxidant activity and bioactive compounds identification of Passiflora subpeltata fruit pulp were investigated. Fifteen polyphenolic compounds including protocatechuic acid, ferulic acid, vanillic acid, epicatechin, p-coumaric acid, cinnamic acid, eriodictyol and quercetin-3-glucoside were identified in the pulp of this species by using UHPLC-QqQ-MS/MS analysis. The total carbohydrates and crude protein contents in fruit pulp were 2.62 mg glucose equivalent/g sample fruit pulp and 8.80 mg BSA equivalent/g sample fruit pulp, respectively. The fresh fruit pulp of P. subpeltata contained high total phenolic (724.76 mg GAE/g sample) content and it revealed very high DPPH (IC50 of 5.667 μg/mL) and ABTS+• (6794.96 μM trolox equivalent/g sample) scavenging activities. In the key enzymes assays useful for diabetic inhibition the fresh fruit pulp characterized maximum inhibition of α-amylase and α-glucosidase IC50 of 18.69 and 32.63 μg/mL, respectively. Thus, these results lead to conclude that this fruit specie could be very useful source in nutraceutical products preparations for Type 2 diabetic suffering humans.



中文翻译:

UHPLC-QqQ-MS / MS鉴定,西番莲果肉中多酚的定量以及营养,抗氧化剂,α-淀粉酶和α-葡萄糖苷酶关键酶抑制特性的测定

研究了西番莲果肉中糖尿病关键酶的抑制,营养,抗氧化活性和生物活性成分的鉴定。通过使用UHPLC-QqQ-MS / MS分析,在该物种的果肉中鉴定出了15种多酚化合物,包括原儿茶酸,阿魏酸,香草酸,表儿茶素,香豆酸,肉桂酸,松香醇和槲皮素-3-葡萄糖苷。果肉中的总碳水化合物和粗蛋白含量分别为2.62 mg葡萄糖当量/ g样品果肉和8.80 mg BSA当量/ g样品果肉。亚庇体育果的新鲜果肉中酚含量较高(724.76 mg GAE / g样品),DPPH (IC5.667μg / mL中的50)和ABTS +•(6794.96μMtrolox当量/ g样品)清除活性。在可用于糖尿病抑制的关键酶分析中,新鲜果肉的特征是最大抑制α-淀粉酶和α-葡萄糖苷酶的IC 50分别为18.69和32.63μg/ mL。因此,这些结果得出结论,该水果可能是用于2型糖尿病患者的营养产品的非常有用的来源。

更新日期:2018-04-04
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