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Identification of macrophage-related candidate genes in lupus nephritis using bioinformatics analysis
Cellular Signalling ( IF 4.8 ) Pub Date : 2018-02-17 , DOI: 10.1016/j.cellsig.2018.02.006
Bingyan Shu , Yi Fang , Weichun He , Junwei Yang , Chunsun Dai

Lupus nephritis (LN) is a chronic autoimmune disorder. Here we try to identify the candidate genes in macrophages related to LN. We performed a systematic search in the Gene Expression Omnibus (GEO) database for microarray in human mononuclear cells and mouse macrophages of LN. The results of clustering and venn analysis of different GEO datasets showed that 8 genes were up-regulated and 2 genes down-regulated in samples from both human and mouse LN. The data from gene network and GO analysis revealed that CD38 and CCL2 were localized in the core of the network. Immunofluorescence staining showed that CD38 expression was markedly increased in macrophages from kidneys with LN. Our study identifies the gene expression profile for macrophages and demonstrated the induction of CCL2 and CD38 in macrophages from patients with LN. However, regarding the limited patient number included in this study, the results are preliminary and more studies are still needed to further decipher the macrophage-related candidate genes for the pathogenesis of LN.



中文翻译:

利用生物信息学分析鉴定狼疮性肾炎中巨噬细胞相关候选基因

狼疮性肾炎(LN)是一种慢性自身免疫性疾病。在这里,我们试图识别与LN相关的巨噬细胞中的候选基因。我们在基因表达综合(GEO)数据库中进行了系统搜索,以查找人单核细胞和LN小鼠巨噬细胞中的微阵列。不同GEO数据集的聚类和维恩分析结果表明,人和小鼠LN样品中有8个基因上调,有2个基因下调。基因网络和GO分析的数据表明,CD38和CCL2位于网络的核心。免疫荧光染色显示,患有LN的肾脏巨噬细胞中CD38表达显着增加。我们的研究确定了巨噬细胞的基因表达谱,并证明了来自LN患者的巨噬细胞中CCL2和CD38的诱导。然而,

更新日期:2018-02-17
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