当前位置: X-MOL 学术Cell. Signal. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
miR-613 inhibits cell migration and invasion by downregulating Daam1 in triple-negative breast cancer
Cellular Signalling ( IF 4.8 ) Pub Date : 2018-01-12 , DOI: 10.1016/j.cellsig.2018.01.013
Huaping Xiong , Ting Yan , Weijie Zhang , Fangfang Shi , Xuesong Jiang , Xiaohua Wang , Shoushan Li , Ying Chen , Cheng Chen , Yichao Zhu

Dishevelled-associated activator of morphogenesis 1 (Daam1) is a formin protein and participates in regulating cell migration of triple-negative breast cancer (TNBC) cells. The specific miRNA targeting Daam1 and mediating cell migration and invasion remains obscure. This experiment investigated the suppressive role of miR-613 in TNBC cells. The luciferase activity of Daam1 3′-untranslated region (3′-UTR) based reporters constructed in HEK-293T and MCF-7 cells suggested that Daam1 was the target gene of miR-613. Overexpressed miR-613 reduced the protein level of Daam1, weakened RhoA activity, and retarded the cell migration, cell invasion and colony formation of TNBC cells. Overexpression of Daam1 or RhoA rescued cell migration and invasion in miR-613-overexpressed TNBC cells, but failed to reverse colony formation. MiR-613 was significantly downregulated in breast cancer tissues compared with that in adjacent normal tissues. This downregulation in TNBC tissues and lymphnode metastatic breast cancer tissues was more obvious than that in non-TNBC tissues and non-metastatic cancer tissues, respectively. MiR-613 weakens the resistance of TNBC cells against paclitaxel rather than adriamycin, cyclophosphamide, docetaxel, and kaempferol. Taken together, miR-613 is involved in cell migration and invasion of TNBC cells via targeting Daam1/RhoA signaling pathway.



中文翻译:

miR-613通过下调Daam1在三阴性乳腺癌中抑制细胞迁移和侵袭

杂散相关的形态发生蛋白1(Daam1)激活剂是一种formin蛋白,参与调节三阴性乳腺癌(TNBC)细胞的细胞迁移。靶向Daam1并介导细胞迁移和侵袭的特定miRNA仍然不清楚。该实验研究了miR-613在TNBC细胞中的抑制作用。基于Daam1 3'-非翻译区(3'-UTR)的报告子在HEK-293T和MCF-7细胞中的荧光素酶活性表明Daam1是miR-613的靶基因。过度表达的miR-613降低了Daam1的蛋白质水平,削弱了RhoA活性,并延迟了TNBC细胞的迁移,侵袭和集落形成。Daam1或RhoA的过表达挽救了miR-613过表达的TNBC细胞中的细胞迁移和侵袭,但未能逆转集落形成。与邻近的正常组织相比,乳腺癌组织中的MiR-613显着下调。TNBC组织和淋巴结转移性乳腺癌组织中的这种下调分别比非TNBC组织和非转移性癌组织中的下调更为明显。MiR-613削弱了TNBC细胞对紫杉醇的抵抗力,而不是对阿霉素,环磷酰胺,多西紫杉醇和山resistance酚的抵抗力。综上所述,miR-613参与了TNBC细胞的细胞迁移和侵袭通过靶向Daam1 / RhoA信号通路。

更新日期:2018-01-12
down
wechat
bug