Bioaugmentation with anaerobic ruminal fungi and a pool of hydrogen-producing fermenting bacteria was tested on wheat straw (WS) and mushroom spent straw (MSS) with the aim of improving anaerobic digestion performance. Batch tests were set up to simulate a Bioaugmentation Anaerobic Digestion (BAD) treatment comparing single- (I-BAD) and two-stage (II-BAD) process configurations, at two reactor scales, 120 and 1200 ml (×10). In both cases, higher CH₄ cumulative production was obtained in the II-BAD configuration on WS (65.1 ± 8.9 Nml and 922 ± 73.8 Nml respectively). The II-BADx10 tests allowed increasing CH₄ production (≃290% and ≃330% on WS and MSS, respectively) when compared to the unaugmented condition. Final results highlighted the achievable advantages of the two stage configuration in terms of CH₄ production enhancement. Microbial community investigations confirmed the efficiency of the bioaugmentation treatment and revealed that such a result was mainly related to the Methanosarcinales increase, mostly composed by Methanosaeta.

为了提高厌氧消化性能，在小麦秸秆（WS）和蘑菇废秸秆（MSS）上测试了厌氧瘤胃真菌和产氢发酵菌池的生物强化作用。设置批处理测试以模拟生物强化厌氧消化（BAD）处理，在两个反应器规模120和1200 ml（×10）下比较单阶段（I-BAD）和两阶段（II-BAD）工艺配置。在这两种情况下，WS上的II-BAD配置均获得了更高的CH ₄累积产量（分别为65.1±8.9 Nml和922±73.8 Nml）。II-BADx10测试允许增加CH ₄与未增强条件相比，产量（分别为WS和MSS的and290％和≃330％）。最终结果凸显了两阶段配置在提高CH ₄产量方面可实现的优势。微生物群落调查证实了生物强化治疗的有效性，并表明该结果主要与甲烷菌的增加有关，主要由甲烷菌组成。