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Real-time fluorescence and deformability cytometry.
Nature Methods ( IF 48.0 ) Pub Date : 2018-04-02 , DOI: 10.1038/nmeth.4639
Philipp Rosendahl 1 , Katarzyna Plak 1, 2 , Angela Jacobi 1 , Martin Kraeter 1, 3 , Nicole Toepfner 1, 4 , Oliver Otto 1 , Christoph Herold 1 , Maria Winzi 1 , Maik Herbig 1 , Yan Ge 1 , Salvatore Girardo 5 , Katrin Wagner 1 , Buzz Baum 2 , Jochen Guck 1
Affiliation  

The throughput of cell mechanical characterization has recently approached that of conventional flow cytometers. However, this very sensitive, label-free approach still lacks the specificity of molecular markers. Here we developed an approach that combines real-time 1D-imaging fluorescence and deformability cytometry in one instrument (RT-FDC), thus opening many new research avenues. We demonstrated its utility by using subcellular fluorescence localization to identify mitotic cells and test for mechanical changes in those cells in an RNA interference screen.



中文翻译:

实时荧光和可变形细胞仪。

细胞机械表征的吞吐量最近已接近传统流式细胞仪的吞吐量。然而,这种非常敏感、无标记的方法仍然缺乏分子标记的特异性。在这里,我们开发了一种将实时一维成像荧光和可变形细胞术结合在一个仪器 (RT-FDC) 中的方法,从而开辟了许多新的研究途径。我们通过使用亚细胞荧光定位来识别有丝分裂细胞并在 RNA 干扰屏幕中测试这些细胞的机械变化来证明其实用性。

更新日期:2018-04-03
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