AbstractA multifunctional fluorescent probe is synthesized for the determination of adenosine 5′-triphosphate (ATP). The 6-carboxyfluorescein-labeled aptamer (FAM-aptamer) was bound to the surface of magnetite nanoparticles coated with polydopamine (Fe3O4@PDA) by π-π stacking interaction to form the multifunctional probe. The probe has three functions including recognition, magnetic separation, and yielding a fluorescent signal. In the presence of ATP, FAM-aptamer on the surface of the probe binds to ATP and returns to the solution. Thus, the fluorescence of the supernatant is enhanced and can be related to the concentration of ATP. Fluorescence intensities were measured at excitation/emission wavelengths of 494/526 nm. Response is linear in the 0.1–100 μM ATP concentration range, and the detection limit is 89 nM. The probe was applied to the quantitation of ATP in spiked human urine and serum samples, with recoveries ranging between 94.8 and 102%. Graphical abstractA multifunctional fluorescent probe based on the use of FAM-aptamer and Fe3O4@PDA is described for the determination of ATP in spiked human urine and serum samples. FAM-aptamer: 6-carboxyfluorescein-labeled aptamer; Fe3O4@PDA: magnetite nanoparticles coated with polydopamine. ATP: adenosine 5′-triphosphate.

中文翻译：

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基于使用标记适体和磁性纳米粒子的多功能探针，用于 5'-三磷酸腺苷的荧光测定

摘要 合成了一种多功能荧光探针，用于测定 5'-三磷酸腺苷 (ATP)。6-羧基荧光素标记的适体（FAM-aptamer）通过π-π堆积相互作用与包覆聚多巴胺（Fe3O4@PDA）的磁铁矿纳米颗粒表面结合形成多功能探针。该探针具有识别、磁分离和产生荧光信号三种功能。在存在 ATP 的情况下，探针表面的 FAM 适配体与 ATP 结合并返回到溶液中。因此，上清液的荧光增强并且可能与 ATP 的浓度有关。在 494/526 nm 的激发/发射波长下测量荧光强度。响应在 0.1–100 μM ATP 浓度范围内呈线性，检测限为 89 nM。该探针用于定量加标人尿液和血清样品中的 ATP，回收率介于 94.8% 和 102% 之间。图形摘要描述了一种基于使用 FAM 适体和 Fe3O4@PDA 的多功能荧光探针，用于测定加标人尿和血清样品中的 ATP。FAM-aptamer：6-羧基荧光素标记的适体；Fe3O4@PDA：聚多巴胺包覆的磁铁矿纳米颗粒。ATP：5'-三磷酸腺苷。涂有聚多巴胺的磁铁矿纳米颗粒。ATP：5'-三磷酸腺苷。涂有聚多巴胺的磁铁矿纳米颗粒。ATP：5'-三磷酸腺苷。