A novel chromatographic material, aminopolycarboxylic-modified silica, was developed by two different synthetic routes, followed by characterization of the prepared material. The methods were compared and discussed based on binding capacity of ligand, chromatographic experiments and metal ion-chelating capacity of the material. Versatility of this chromatographic material including ion-exchange and affinity behaviors for proteins as well as chelating property for metal ions were investigated. The results showed that the material was prepared successfully, and synthetic method II was more favorable to the preparation of the material. This novel chromatographic material displayed good chromatographic behaviors for the separation of proteins, as well as outstanding chelating property for metal ions. Versatility of the material can effectively expand the applications of this functional material in different research fields.

通过两种不同的合成途径开发了一种新型色谱材料，氨基聚羧酸改性的二氧化硅，然后表征了所制备的材料。根据配体的结合能力，色谱实验和材料的金属离子螯合能力对方法进行了比较和讨论。研究了这种色谱材料的多功能性，包括离子交换和对蛋白质的亲和行为以及对金属离子的螯合性能。结果表明该材料制备成功，合成方法Ⅱ更有利于该材料的制备。这种新颖的色谱材料显示出良好的色谱行为，可分离蛋白质，以及出色的金属离子螯合性能。