In the biorefinery concept renewable feedstocks are converted to a multitude of value-added compounds irrespective of seasonal or other variations of the complex biomass substrates. Conceptionally, this can be realized by specialized single microbial strains or by co-culturing various strain combinations. In the latter approach strains for substrate conversion and for product formation can be combined. This study addressed the construction of binary microbial consortia based on starch- and sucrose-based production of l-lysine and derived value-added compounds. A commensalism-based synthetic consortium for l-lysine production from sucrose was developed combining an l-lysine auxotrophic, naturally sucrose-negative E. coli strain with a C. glutamicum strain able to produce l-lysine that secretes fructose when grown with sucrose due to deletion of the fructose importer gene ptsF. Mutualistic synthetic consortia with an l-lysine auxotrophic, α-amylase secreting E. coli strain and naturally amylase-negative C. glutamicum strains was implemented for production of valuable fine chemicals from starch.

在生物精炼概念中，可再生原料被转换为多种增值化合物，而与复杂生物质底物的季节或其他变化无关。从概念上讲，这可以通过专门的单一微生物菌株或通过共培养各种菌株组合来实现。在后一种方法中，可以组合用于底物转化和用于产物形成的菌株。本研究涉及的二进制微生物聚基于淀粉和蔗糖为基础的生产的结构1-赖氨酸和派生增值化合物。对于基于共栖合成财团-1-从蔗糖赖氨酸生产的开发相结合的1-赖氨酸营养缺陷型，自然蔗糖阴性大肠杆菌具有应变谷氨酸棒杆菌菌株能够产生升时与蔗糖中生长，由于果糖进口商基因的缺失赖氨酸分泌果糖PTSF。与互惠合成财团1-赖氨酸营养缺陷型，α淀粉酶分泌的大肠杆菌菌株和天然淀粉酶阴性的谷氨酸棒杆菌菌株中生产的由淀粉有价值精细化学品来实现。