Abstract The objective of this study was to study the effect of calcium ions on the binding of lysozyme to chitosan (CS)/sodium alginate (SA) complexes (CS-SA-Ps), in order to develop a complex system for lysozyme delivery which might be of practical interest in the field of food preservatives. Result showed that with the increase of calcium ions (Ca2+) concentration, the value of Z-Ave decreased while the value of ζ-potential and LE increased, where the addition of 5.0 mM Ca2+ contributed to uniform distribution of the complexes. Sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) revealed that there was no significant difference of molecular integrity when lysozyme was released from CS-SA-Ps. The exothermic interaction between lysozyme and SA were proved by thermodynamic measurements, in which the binding stoichiometry showed a slight decrease in the presence of calcium chloride. Moreover, the release rate of lysozyme from CS-SA-Ps could be slowed by the increase of Ca2+ addition concentration.

中文翻译：

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负载溶菌酶的海藻酸钙-壳聚糖复合物的制备与表征

摘要 本研究的目的是研究钙离子对溶菌酶与壳聚糖 (CS)/海藻酸钠 (SA) 复合物 (CS-SA-Ps) 结合的影响，以开发一种复杂的溶菌酶递送系统。可能在食品防腐剂领域具有实际意义。结果表明，随着钙离子（Ca2+）浓度的增加，Z-Ave 值降低，而 ζ-电位和 LE 值增加，其中加入 5.0 mM Ca2+ 有助于复合物的均匀分布。硫酸盐-聚丙烯酰胺凝胶电泳 (SDS-PAGE) 显示当溶菌酶从 CS-SA-Ps 释放时，分子完整性没有显着差异。通过热力学测量证明了溶菌酶和 SA 之间的放热相互作用，其中结合化学计量显示在氯化钙的存在下略有下降。此外，CS-SA-Ps 溶菌酶的释放速率会随着 Ca2+ 添加浓度的增加而减慢。