Nepafenac is novel ophthalmic agents with a unique prodrug structure and superior corneal permeability to other currently available topical nonsteroidal anti-inflammatory drugs. A graphene nanoplatelets and carbon nanofibers modified glassy carbon electrode was developed and used as first electrochemical sensor for the determination of nepafenac. The effective combination of graphene and carbon nanofibers resulted in electrochemical sensor with large surface area, high sensitivity, good stability and selectivity. After method optimization, the square wave voltammograms, at a preconcentration time of 60 s, display two linear ranges of 4.0 × 10⁻⁶–1.5 × 10⁻⁵ M and 2.5 × 10⁻⁷–4.0 × 10⁻⁶ M for drug quantification with a detection limit of 6.3 × 10⁻⁸ M. The modified electrode was employed for fast assay of nepafenac in ophthalmic solution with satisfactory results and rapid run time of 66 s in comparison with previously reported analytical methods.

Nepafenac是新型的眼药，具有独特的前药结构和比其他目前可用的局部非甾体类抗炎药优越的角膜通透性。开发了石墨烯纳米片和碳纳米纤维修饰的玻碳电极，并将其用作测定奈帕芬酸的首个电化学传感器。石墨烯和碳纳米纤维的有效结合导致电化学传感器具有大的表面积，高的灵敏度，良好的稳定性和选择性。经过方法优化后，在60 s的预浓缩时间下，方波伏安图显示两个线性范围，分别为4.0×10 ^-6 –1.5×10 ^-5  M和2.5×10 ^-7 –4.0×10 ^-6 M用于药物定量，检测极限为6.3×10 ^-8M  。与以前报道的分析方法相比，该修饰的电极可快速测定眼药水中的奈帕芬酸，具有令人满意的结果，并且快速运行时间为66 s。