In this study, the protection effect of black carrot extract on the stability of liposomes during storage was evaluated. The physical and chemical stability of the extract (0.4% w/w) including extract-loaded liposomes with various lecithin content (1%, 2%, and 4% w/w) were demonstrated. Z-average particle diameter and zeta potentials of liposomes were evaluated before and after 21 days of storage. The particle diameter of the samples was found to be lower than 50 nm after storage and no statistical change was determined (p < 0.05). The liposomes with and without extract were visualized by transmission electron microscopy (TEM) after negative staining. The TEM images revealed that unloaded and extract-loaded liposomes are similar in size. The degradation of extract (30%–90%), phenolic content (10%–29%), and antioxidant capacity (4%–33%) of liposomes depending on lecithin content were also determined during storage to evaluate the biochemical stability. A hexanal analysis was performed to investigate the lipid oxidation in liposomes within 60 days. According to the results, the oxidation of polyunsaturated fatty acids may be inhibited with the addition of the extract to liposomes; however, lipid content should be limited according to the extract concentration. The highest protection on oxidation was observed in extract-loaded liposomes containing 1% lecithin. The study provided valuable data on the contribution of an anthocyanin addition to liposomes to overcome oxidation of unsaturated phospholipids.

在这项研究中，评估了黑胡萝卜提取物对脂质体在储存过程中的稳定性的保护作用。证明了提取物的物理和化学稳定性（0.4％w / w），包括具有各种卵磷脂含量（1％，2％和4％w / w）的负载提取物的脂质体。ž在储存21天之前和之后评估脂质体的平均粒径和ζ电势。储存后发现样品的粒径小于50 nm，未发现统计学变化（p <0.05）。在阴性染色后，通过透射电子显微镜（TEM）观察具有和不具有提取物的脂质体。TEM图像显示，未负载和提取物负载的脂质体大小相似。根据卵磷脂含量，还确定了脂质体提取物的降解（30％–90％），酚含量（10％–29％）和抗氧化能力（4％–33％），以评估其生化稳定性。进行了己醛分析以研究60天内脂质体中的脂质氧化。根据结果​​，通过将提取物添加到脂质体中，可以抑制多不饱和脂肪酸的氧化；但是，应根据提取物的浓度限制脂质含量。在含有1％卵磷脂的提取物负载的脂质体中观察到最高的抗氧化保护作用。该研究提供了有关花青素除脂质体克服不饱和磷脂氧化作用方面的有价值的数据。