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Highly sensitive detection of a small molecule by a paired labels recognition system based lateral flow assay
Analytical and Bioanalytical Chemistry ( IF 4.3 ) Pub Date : 2018-03-28 , DOI: 10.1007/s00216-018-1003-0
Leina Dou , Bingxin Zhao , Tong Bu , Wentao Zhang , Qiong Huang , Lingzhi Yan , Lunjie Huang , Yanru Wang , Jianlong Wang , Daohong Zhang

Small molecules are difficult to detect by conventional gold lateral flow assay (GLFA) sensitively because the test system must satisfy the conflict requirements between enough signal intensity and limited antibody (Ab) amount. In this work, a paired labels recognition (PLR)-based biosensor was designed by utilizing the specific binding of Ab and secondary antibody (anti-Ab) to enhance signal intensity and reduce antibody amount applied in small molecule detection. The PLR amplification system is fabricated by self-assembling the common detection probe, Au-labeled Ab (Au-Ab), and the signal booster, Au-labeled anti-Ab (Au-anti-Ab). Benefiting from this, a powerful network structure can be generated to accumulate numerous gold nanoparticles (GNPs) and thus significantly strengthen the signal intensity of detection. Therefore, a lower Ab amount will be applied to offer adequate signal strength, and further, the limit of detection will be obviously downregulated due to the more effective competition reaction. Using furazolidone (FZD) as a model analyte, we achieve a detection limit of as low as 1 ng mL−1, which was at least fivefold improved over that of the traditional GLFA. Furthermore, the practicality of this strategy was certificated in five different food samples.

Open image in new windowGraphical abstract
Graphical abstract

A paired labels recognition (PLR) amplification system is fabricated by self-assembling the common detection probe, Au-labeled Ab (Au-Ab), and the signal booster, Au-labeled anti-Ab (Au-anti-Ab). In this novel strategy, owing to the recognition of both Ab and anti-Ab labeled on gold nanoparticles (GNPs), a powerful network structure can be generated to accumulate numerous GNPs and thus significantly strengthen the signal intensity of detection.



中文翻译:

通过基于侧向流动分析的配对标记识别系统对小分子进行高度灵敏的检测

小分子很难通过常规的金侧流测定法(GLFA)灵敏地检测出来,因为测试系统必须满足足够的信号强度和有限的抗体(Ab)量之间的冲突要求。在这项工作中,通过利用抗体和二抗(抗抗体)的特异性结合来设计基于配对标记识别(PLR)的生物传感器,以增强信号强度并减少在小分子检测中应用的抗体量。通过自组装通用检测探针Au标记的Ab(Au-Ab)和信号增强器Au标记的抗Ab(Au-anti-Ab),可以组装PLR放大系统。得益于此,可以生成强大的网络结构来积累大量金纳米颗粒(GNP),从而显着增强检测信号强度。所以,较低的Ab量将被应用以提供足够的信号强度,此外,由于更有效的竞争反应,检测限将明显下调。使用呋喃唑酮(FZD)作为模型分析物,我们的检测限可低至1 ng mL-1,比传统GLFA至少提高了五倍。此外,在五个不同的食物样本中证明了该策略的实用性。

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图形概要

通过自组装通用检测探针Au标记的Ab(Au-Ab)和信号增强器Au标记的抗Ab(Au-anti-Ab),可以组装成对的标记识别(PLR)扩增系统。在这种新颖的策略中,由于识别了金纳米颗粒(GNP)上标记的Ab和抗Ab,因此可以生成强大的网络结构来积累大量GNP,从而显着增强检测的信号强度。

更新日期:2018-03-28
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