An artificial metabolic route to an unnatural trichothecene was designed by taking advantage of the broad substrate specificities of the T-2 toxin biosynthetic enzymes of Fusarium sporotrichioides. By feeding 7-hydroxyisotrichodermin, a shunt pathway metabolite of F. graminearum, to a trichodiene synthase-deficient mutant of F. sporotrichioides, 7-hydroxy T-2 toxin (1) was obtained as the final metabolite. Such an approach may have future applications in the metabolic engineering of a variety of fungal secondary metabolites. The toxicity of 7-hydroxy T-2 toxin was 10 times lower than that of T-2 toxin in HL-60 cells.

中文翻译：

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探索镰孢镰刀菌中的人工代谢途径：7羟基T-2毒素的产生和表征

利用镰刀镰刀菌的T-2毒素生物合成酶的广泛底物特异性，设计了一种人工代谢途径来合成非天然的上孢烯。通过将禾本科镰刀菌的分流途径代谢物7-羟基异麦角菌素喂入孢子孢子虫的三甲二烯合酶缺陷型突变体，获得了7-羟基T-2毒素（1）作为最终代谢产物。这种方法可能在各种真菌次生代谢产物的代谢工程中具有未来的应用。7-羟基T-2毒素在HL-60细胞中的毒性比T-2毒素低10倍。