In this work, we demonstrate, for the first time, the development of a disposable MoS₂-arrayed matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) chip combined with an immunoaffinity enrichment method for high-throughput, rapid, and simultaneous quantitation of multiple sulfonamides (SAs). The disposable MALDI MS chip was designed and fabricated by MoS₂ array formation on a commercial indium tin oxide (ITO) glass slide. A series of SAs were analyzed, and clear deprotonated signals were obtained in negative-ion mode. Compared with MoS₂-arrayed commercial steel plate, the prepared MALDI MS chip exhibited comparable LDI efficiency, providing a good alternative and disposable substrate for MALDI MS analysis. Furthermore, internal standard (IS) was previously deposited onto the MoS₂ array to simplify the experimental process for MALDI MS quantitation. 96 sample spots could be analyzed within 10 min in one single chip to perform quantitative analysis, recovery studies, and real foodstuff detection. Upon targeted extraction and enrichment by antibody conjugated magnetic beads, five SAs were quantitatively determined by the IS-first method with the linear range of 0.5–10 ng/mL (R² > 0.990). Good recoveries and repeatability were obtained for spiked pork, egg, and milk samples. SAs in several real foodstuffs were successfully identified and quantified. The developed method may provide a promising tool for the routine analysis of antibiotic residues in real samples.

中文翻译：

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一次性MoS ₂阵列MALDI MS芯片用于高通量和快速定量多个真实样品中的磺酰胺

在这项工作中，我们首次展示了结合了免疫亲和富集方法的一次性MoS ₂阵列基质辅助激光解吸/电离质谱（MALDI MS）芯片的开发，可实现高通量，快速和同时多种磺酰胺（SA）的定量分析。一次性MALDI MS芯片是通过在商业铟锡氧化物（ITO）载玻片上形成MoS ₂阵列来设计和制造的。分析了一系列SA，并在负离子模式下获得了清晰的去质子化信号。与MoS ₂相比阵列排列的商用钢板，制得的MALDI MS芯片表现出可比的LDI效率，为MALDI MS分析提供了良好的替代性和一次性基材。此外，预先将内标（IS）沉积在MoS ₂阵列上，以简化MALDI MS定量的实验过程。可以在10分钟内用一个芯片对96个样品点进行分析，以进行定量分析，回收率研究和实际食品检测。经抗体结合的磁珠靶向提取和富集后，采用IS-first方法定量测定了5个SA，线性范围为0.5-10 ng / mL（R ²> 0.990）。加标猪肉，鸡蛋和牛奶样品的回收率和重复性良好。成功鉴定和定量了几种真实食品中的SA。所开发的方法可能为常规分析实际样品中的抗生素残留提供有希望的工具。