Malachite green (MG) is an industrial dye, but has been once abused in aquaculture for disease treatment. Because MG and its metabolite, leucomalachite green (LMG), present great harm to human health, it is essential to rapidly and accurately detect MG and LMG residues in aquatic products. However, the simultaneous screening of MG and LMG is still a challenge to date. In this work, a dual-template molecularly imprinted polymer (MIP) coated CdTe QDs was developed and applied as a nano-probe to simultaneously detect MG and LMG in fish samples via normal and synchronous fluorescence quenching. The molar ratios of templates (MG and LMG) to monomer (APTES) and cross-linker (TEOS), and the amount of CdTe QDs in the probe preparation were optimized. A normal fluorescence emission at 600 nm could be linearly quenched by MG, while the synchronous fluorescence emission at the wavelength interval of 340 nm could be quenched by both LMG and MG. The detection limits of MG and LMG were 17 nmol L⁻¹ and 28 nmol L⁻¹ (3σ, n = 9), respectively. Based on the equations of Stern-Volmer, MG and LMG could be simultaneously detected without any conversion treatment. The recoveries of MG and LMG in fish samples ranged from 92% to 107% and from 91% to 108%, respectively. The results demonstrated that the as-prepared dual-template [email protected] could be used as a fluorescent probe to rapidly and simultaneously detect MG and LMG in fish samples.

孔雀石绿（MG）是一种工业染料，但曾经在水产养殖中被滥用以治疗疾病。由于MG及其代谢物白孔雀绿（LMG）对人体健康具有极大危害，因此必须快速，准确地检测水产品中的MG和LMG残留。然而，迄今为止，同时筛查MG和LMG仍然是一个挑战。在这项工作中，开发了一种双模板分子印迹聚合物（MIP）包覆的CdTe QD，并作为纳米探针应用，可通过正常和同步荧光淬灭同时检测鱼样品中的MG和LMG。优化了探针制备中模板（MG和LMG）与单体（APTES）和交联剂（TEOS）的摩尔比以及CdTe QD的数量。MG可以线性淬灭600 nm处的正常荧光发射，LMG和MG可以猝灭340 nm波长间隔的同步荧光。MG和LMG的检出限为17 nmol L^-1和28 nmol L ^-1（3σ，n = 9）。根据Stern-Volmer方程，无需进行任何转换处理就可以同时检测MG和LMG。鱼样品中MG和LMG的回收率分别为92％至107％和91％至108％。结果表明，所制备的双模板（受电子邮件保护）可以用作荧光探针，以快速，同时检测鱼样品中的MG和LMG。