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Nanostructuring Lipophilic Dyes in Water Using Stable Vesicles, Quatsomes, as Scaffolds and Their Use as Probes for Bioimaging
Small ( IF 13.3 ) Pub Date : 2018-03-24 , DOI: 10.1002/smll.201703851
Antonio Ardizzone 1 , Siarhei Kurhuzenkau 2 , Sílvia Illa‐Tuset 3 , Jordi Faraudo 1 , Mykhailo Bondar 4 , David Hagan 5 , Eric W. Van Stryland 5 , Anna Painelli 2 , Cristina Sissa 2 , Natalia Feiner 6 , Lorenzo Albertazzi 6 , Jaume Veciana 1 , Nora Ventosa 1
Affiliation  

A new kind of fluorescent organic nanoparticles (FONs) is obtained using quatsomes (QSs), a family of nanovesicles proposed as scaffolds for the nanostructuration of commercial lipophilic carbocyanines (1,1′‐dioctadecyl‐3,3,3′,3′‐tetramethyl‐indocarbocyanine perchlorate (DiI), 1,1′‐dioctadecyl‐3,3,3′,3′‐tetramethyl‐indodicarbocyanine perchlorate (DiD), and 1,1′‐dioctadecyl‐3,3,3′,3′‐tetramethyl‐indotricarbocyanine iodide (DiR)) in aqueous media. The obtained FONs, prepared by a CO2‐based technology, show excellent colloidal‐ and photostability, outperforming other nanoformulations of the dyes, and improve the optical properties of the fluorophores in water. Molecular dynamics simulations provide an atomistic picture of the disposition of the dyes within the membrane. The potential of QSs for biological imaging is demonstrated by performing superresolution microscopy of the DiI‐loaded vesicles in vitro and in cells. Therefore, fluorescent QSs constitute an appealing nanomaterial for bioimaging applications.

中文翻译:

使用稳定的囊泡,四倍体作为支架,并将其用作生物成像探针,对水中的亲脂性染料进行纳米结构化。

使用季体(QSs)获得了一种新型的荧光有机纳米颗粒(FONs),季铵盐是一类纳米囊泡,被提议用作商用亲脂性花青素(1,1'-dioctadecyl-3,3,3',3'-的纳米结构的支架)高碘酸四甲基-吲哚羰花青(DiI),高氯酸1,1'-二十八碳三酯,3,3',3'-四甲基-茚二甲酸花青酸高氯酸盐(DiD)和1,1'-二十八碳氰化花青-3,3,3',3' -在水性介质中的四甲基-吲哚并呋喃碘化物(DiR))。由CO 2制备的所得FON基于这项技术,具有出色的胶体和光稳定性,优于染料的其他纳米配方,并改善了荧光团在水中的光学性能。分子动力学模拟提供了染料在膜内布置的原子图。通过在体外和细胞中对载有DiI的囊泡进行超高分辨率显微镜检查,证明了QSs在生物成像中的潜力。因此,荧光QSs构成了用于生物成像应用的吸引人的纳米材料。
更新日期:2018-03-24
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