AbstractColloidal gold, quantum dots and polystyrene microspheres were used as labels in three kinds of lateral flow immunochromatographic assays (ICAs) for the detection of zearalenone (ZEN) in cereal samples. The assays allow ZEN to be quantified within 20 min. The LODs are 10 μg·L−1 of ZEN for the colloidal gold-based ICA, and 1 μg·L−1 for both the quantum dot and polystyrene microsphere based ICAs. The respective data are 60 μg·kg−1, 6 μg·kg−1 and 6 μg·kg−1, respectively, for spiked samples and cereals. Only minor cross-sensitivity occurred between ZEN and fusarium toxins, and no cross-sensitivity if found for aflatoxin B1, T-2 mycotoxin, ochratoxin A, deoxynivalenol, and fumonisin B1. LODs of the three assays are lower than the maximum limits of ZEN set by most standardization agencies. Graphical abstractSchematic presentation of three lateral flow immunochromatographic assays (ICAs) based on the use of (a) colloidal gold (CG), (b) fluorescent quantum dots (QD), and (c) polystyrene microspheres (PMs) as signalling labels for the rapid and sensitive determination of zearalenone (ZEN) in cereal samples.

中文翻译：

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基于纳米颗粒标记的三种侧流免疫层析测定法用于玉米赤霉烯酮的荧光测定

摘要 以胶体金、量子点和聚苯乙烯微球为标记，在三种横向流动免疫层析法（ICA）中检测谷物样品中的玉米赤霉烯酮（ZEN）。该检测允许在 20 分钟内对 ZEN 进行定量。胶体金基 ICA 的 LOD 为 10 μg·L-1 ZEN，量子点和聚苯乙烯微球基 ICA 的 LOD 为 1 μg·L-1。加标样品和谷物的相应数据分别为 60 μg·kg-1、6 μg·kg-1 和 6 μg·kg-1。ZEN 与镰刀菌毒素之间仅发生轻微交叉敏感性，如果发现黄曲霉毒素 B1、T-2 霉菌毒素、赭曲霉毒素 A、脱氧雪腐镰刀菌烯醇和伏马菌素 B1，则无交叉敏感性。三种检测的 LOD 均低于大多数标准化机构设定的 ZEN 的最大限值。