Melissa officinalis, Lavandula latifolia and Origanum vulgare are widely used medicinal plants and spices. Their extracts were evaluated as potential antioxidants for functional food formulations. After being submitted to an in vitro gastrointestinal digestion, all the extracts showed antioxidant activity (measured by DPPH, ABTS, FRAP, TPC). Furthermore, their main polyphenols maintained high stability. Biological activity was measured using Caenorhabditis elegans, which is a widely used model in this context. Treatments of 50 and 100 µg/mL of M. officinalis significantly attenuated juglone-induced stress in the survival assay performed; moreover, all tested concentrations decreased intracellular ROS. Lavandula latifolia and O. vulgare had no significant effect against acute stress in the survival assay, but significantly decreased ROS basal levels. GST-4 expression under juglone-induced oxidative stress was significantly down-regulated by treatment with the three plant extracts (up to 63%). Besides, similar biological activity of all digested extracts was demonstrated in all in vivo assays.

蜜蜂花（Melissa officinalis），薰衣草（Lavandula latifolia）和牛至（Origanum vulgare）是广泛使用的药用植物和香料。他们的提取物被评估为功能性食品配方的潜在抗氧化剂。经过体外胃肠消化后，所有提取物均显示出抗氧化活性（通过DPPH，ABTS，FRAP，TPC测定）。此外，它们的主要多酚保持高稳定性。使用秀丽隐杆线虫（Caenorhabditis elegans）测量生物学活性，秀丽隐杆线虫是在这种情况下被广泛使用的模型。处理50和100 µg / mL的厚朴在进行的存活测定中显着减轻了juglone诱导的压力；此外，所有测试浓度均降低了细胞内ROS。薰衣草和小叶O. vulgare在存活试验中对急性应激没有明显影响，但是显着降低了ROS的基础水平。通过三种植物提取物的处理，在juglone诱导的氧化应激下，GST-4的表达显着下调（高达63％）。此外，在所有体内测定中都证明了所有消化提取物的相似生物活性。