The objective of this study was to investigate the effect of complexation of epigallocatechin-gallate (EGCG) to a caseinate-stabilized oil-water interface on the bioefficacy of EGCG. The bioaccessibility, anti-proliferative activity, and intestinal uptake of EGCG are reported. Higher amount of EGCG was present in digested emulsions compared to their equivalent solutions after digestion. The incorporation of EGCG in sodium caseinate emulsions improved its bioaccesibility and this was confirmed by cytotoxicity assays on Caco-2 cells. Intestinal absorption of EGCG was also studied with in vitro transport experiments. It was difficult to obtain a quantitative measurement of the EGCG in the basolateral fraction, but the fractions showed a clear anti-proliferative activity. These results would suggest the use of a cytotoxicity assay on cell cultures to estimate the extent of intestinal absorption of the bioactive catechin EGCG. The findings demonstrated that sodium caseinate-stabilized emulsions can be used as a platform for delivery of EGCG.

这项研究的目的是调查表没食子儿茶素-没食子酸酯（EGCG）络合到酪蛋白酸盐稳定的油水界面对EGCG的生物功效的影响。报道了EGCG的生物可及性，抗增殖活性和肠道吸收。与消解后的同等溶液相比，消解的乳剂中存在更多的EGCG。在酪蛋白酸钠乳剂中掺入EGCG改善了其生物适应性，这一点已通过对Caco-2细胞的细胞毒性试验得以证实。还通过体外研究了EGCG的肠道吸收运输实验。难以获得基底外侧部分中EGCG的定量测量结果，但是这些部分显示出明显的抗增殖活性。这些结果将暗示在细胞培养物中使用细胞毒性测定法来估计生物活性儿茶素EGCG的肠道吸收程度。这些发现证明酪蛋白酸钠稳定的乳剂可以用作EGCG的递送平台。