Prickly pear is an important source of bioactive compounds. However, a comprehensive characterization of the phytochemical profile of its aerial botanical parts, considering genotypic differences, has not been conducted. This study evaluated the phytochemical composition of four botanical parts (fruit pulp and skin, and young and adult cladodes) of six cultivars. Analysis was carried out by using two non-targeted UHPLC-ESI-MSⁿ experimental conditions and assisted with multivariate analysis to facilitate data interpretation. Up to 41 compounds, mainly (poly)phenolic molecules, were identified and quantified, 23 compounds being reported for the first time in Opuntia ficus-indica. Phenolic composition varied significantly depending on the part of the plant. Betalains were detected only in the fruit of a red cultivar. This study provided novel insights in terms of identification of bioactives and thorough characterization of botanical parts of prickly pears. This information may be used for the development of prickly pear-derived products with high levels of bioactive compounds.

花椒是生物活性化合物的重要来源。但是，考虑到基因型差异，尚未对其空中植物部分的植物化学特征进行全面表征。这项研究评估了六个品种的四个植物部分（果肉和皮肤，以及年轻的和成年的枝条）的植物化学组成。通过使用两个非目标UHPLC-ESI-MS ⁿ实验条件进行分析，并辅以多变量分析以促进数据解释。鉴定和定量了多达41种化合物，主要是（多）酚类分子，其中23种化合物首次在仙人掌仙人掌中报道。。酚类成分随植物部位的不同而有很大差异。仅在红色品种的果实中检测到甜菜碱。这项研究为刺梨的生物活性成分鉴定和植物部位的彻底表征提供了新颖的见解。该信息可用于开发具有高水平生物活性化合物的刺梨衍生产品。