Information on drug transfer into the breast milk is essential to protect the infant from undesirable adverse effects of maternal consumption of drugs and to allow effective pharmacological treatment of breastfeeding mothers. Metronidazole and fluconazole are two drugs frequently used in nursing women to treat various infections, thus questioning infant's safety due to drug exposure through breast milk. In this article a porous graphitized carbon LC/ESI-MS assay was developed for the quantitation of metronidazole and fluconazole in breast milk and human plasma. The assay was based on the use of 150 μL of biological samples, following acetonitrile precipitation of proteins and filtration that enabled injection into the LC/ESI-MS system. All analytes and the internal standard, ropinirole, were separated by using a porous graphitized carbon analytical column (150 × 2.1 mm i.d., particle size 5 μm) with isocratic elution. The mobile phase consists of 55% acetonitrile in water acidified with 0.1% concentrated formic acid and pumped at a flow rate of 0.25 mL min⁻¹. The assay was linear over a concentration range of 0.1 to 15 μg mL⁻¹ for all analytes in both biological samples. Intermediate precision was found to be <8.4% over the tested concentration ranges. A run time of <5 min for each sample made it possible to analyze a large number of biological samples per day. The method is the first reported application for the analysis of metronidazole and fluconazole in both breast milk and human plasma and it can be used to support a wide range of clinical studies.

有关将药物转移到母乳中的信息对于保护婴儿免受母体药物消费的不良不良影响并允许对母乳喂养的母亲进行有效的药理治疗是必不可少的。甲硝唑和氟康唑是护理妇女中经常使用的两种药物，用于治疗各种感染，因此由于母乳中的药物接触，婴儿的安全性受到质疑。在本文中，开发了一种多孔石墨化碳LC / ESI-MS测定法，用于定量母乳和人血浆中的甲硝唑和氟康唑。该测定基于使用150μL的生物样品，然后乙腈沉淀蛋白质并进行过滤，使其能够进样到LC / ESI-MS系统中。所有分析物和内标罗匹尼罗 使用多孔石墨化碳分析柱（内径150×2.1 mm，粒径5μm）进行分离，并进行等度洗脱。流动相由50.1％的甲酸酸化的水中的55％乙腈组成，并以0.25 mL / min的流速泵送^-1。对于两种生物样品中的所有分析物，该测定在0.1至15μgmL ^-1的浓度范围内都是线性的。发现在测试的浓度范围内，中间精度<8.4％。每个样品的运行时间少于5分钟，因此每天可以分析大量生物样品。该方法是首次报告的用于分析母乳和人血浆中甲硝唑和氟康唑的方法，可用于支持广泛的临床研究。