Microprojection array (MPA) patches are an attractive approach to selectively capture circulating proteins from the skin with minimal invasiveness for diagnostics at the point-of-care or in the home. A key challenge to develop this technology is to extract sufficient quantities of specific proteins from within the skin to enable high diagnostic sensitivity within a convenient amount of time. To achieve this, we investigated the effect of MPA geometry (i.e. projection density, length and array size) on protein capture. We hypothesised that the penetrated surface area of MPAs is a major determinant of protein capture however it was not known if simultaneously increasing projection density, length and array size is possible without adversely affecting penetration and/or tolerability. We show that increasing the projection density (5,000 - 30,000 proj.cm^-2) and array size (4 – 36 mm²) significantly increases biomarker capture whilst maintaining of a similar level tolerability, which supports previous literature for projection length (40 – 190 µm). Ultimately, we designed a high surface area MPA (30,000 proj.cm^-2, 36 mm², 140 µm) with a 4.5-fold increase in penetrated surface area compared to our standard MPA design (20,408 proj.cm^-2, 16 mm², 100 µm). The high surface area MPA captured antigen-specific IgG from mice in 30 s with 100% diagnostic sensitivity compared with 10 - 30 min for previous MPA immunoassay patches, which is over an order of magnitude reduction in wear time. This demonstrates for the first time that MPAs may be used for ultra-rapid (< 1 min) protein capture from skin in a time competitive with standard clinical procedures like the needle and lancet, which has broad implications for minimally invasive and point-of-care diagnostics.

微突出物阵列（MPA）贴片是一种吸引人的方法，可以以最小的侵入性从皮肤上选择性地捕获循环蛋白，以便在现场或在家中进行诊断。开发该技术的关键挑战是从皮肤内提取足够数量的特定蛋白质，以在方便的时间内实现较高的诊断敏感性。为此，我们研究了MPA几何形状（即投影密度，长度和阵列大小）对蛋白质捕获的影响。我们假设MPA的穿透表面积是蛋白质捕获的主要决定因素，但是尚不清楚是否可以同时增加投影密度，长度和阵列大小而不会不利地影响穿透和/或耐受性。我们显示出增加投影密度（5,000-30,000 proj。^-2）和阵列大小（4 – 36 mm ²）显着增加了生物标记物的捕获，同时保持了相似的水平耐受性，这支持了有关投影长度（40 – 190 µm）的先前文献。最终，我们设计了一个高表面积的MPA（30,000 proj.cm ^-2，36毫米²，140μm），以在穿透的表面积的4.5倍的增加相比，我们的标准MPA设计（20408 proj.cm ^-2，16毫米^2个，100 µm）。高表面积的MPA在30 s内以100％的诊断敏感性从小鼠中捕获了抗原特异性IgG，而以前的MPA免疫测定贴片则需要10-30分钟，这在磨损时间上减少了一个数量级。这首次证明了MPA可用于在与标准临床程序（例如针头和刺血针）竞争的时间内，从皮肤中超快速（<1分钟）捕获蛋白质，这对微创和切点具有广泛的意义。护理诊断。