Aptamer-based paper strip sensor for detecting Vibrio fischeri was developed. Our method was based on the aptamer sandwich assay between whole live cells, V. fischeri and DNA aptamer probes. Following 9 rounds of Cell-SELEX and one of the negative-SELEX, V. fischeri Cell Aptamer (VFCA)-02 and -03 were isolated, with the former showing approximately 10-fold greater avidity (in the subnanomolar range) for the target cells when arrayed on a surface. The colorimetric response of a paper sensor based on VFCA-02 was linear in the range of 4 × 10¹ to 4 × 10⁵ CFU/mL of target cell by using scanning reader. The linear regression correlation coefficient (R²) was 0.9809. This system shows promise for use in aptamer-conjugated gold nanoparticle probes in paper strip format for in-field detection of marine bioindicating bacteria.

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基于适体的纸条传感器，用于检测费氏弧菌

开发了基于适体的纸条传感器，用于检测费氏弧菌。我们的方法基于整个活细胞，费氏弧菌和DNA适体探针之间的适体夹心测定。下列9轮细胞SELEX的和负SELEX的一个V.鲵细胞适体（VFCA）-02和-03分离，前者示出约10倍的亲合力更大（在亚纳摩尔范围内），用于在目标细胞排列在表面上时。使用扫描仪，基于VFCA-02的纸张传感器的比色响应在目标细胞的4×10 ¹至4×10 ⁵ CFU / mL范围内呈线性。线性回归相关系数（R ²）为0.9809。该系统显示了以纸带形式用于适体缀合的金纳米颗粒探针的前景，可用于海洋生物指示细菌的现场检测。