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Analysis of oscillatory rocking curve by dynamical diffraction in protein crystals [Physics]
Proceedings of the National Academy of Sciences of the United States of America ( IF 11.1 ) Pub Date : 2018-04-03 00:00:00 , DOI: 10.1073/pnas.1720098115
Ryo Suzuki 1 , Haruhiko Koizumi 2 , Keiichi Hirano 3 , Takashi Kumasaka 4 , Kenichi Kojima 5 , Masaru Tachibana 1
Affiliation  

High-quality protein crystals meant for structural analysis by X-ray diffraction have been grown by various methods. The observation of dynamical diffraction in protein crystals is an interesting topic because dynamical diffraction generally occurs in perfect crystals such as Si crystals. However, to our knowledge, there is no report yet on protein crystals showing clear dynamical diffraction. We wonder whether the perfection of protein crystals might still be low compared with that of high-quality Si crystals. Here, we present observations of the oscillatory profile of rocking curves for protein crystals such as glucose isomerase crystals. The oscillatory profiles are in good agreement with those predicted by the dynamical theory of diffraction. We demonstrate that dynamical diffraction occurs even in protein crystals. This suggests the possibility of the use of dynamical diffraction for the determination of the structure and charge density of proteins.



中文翻译:

蛋白质晶体中动态衍射的振荡摇摆曲线分析[物理]

用于X射线衍射结构分析的高质量蛋白质晶体已经通过各种方法生长。蛋白质晶体中动态衍射的观察是一个有趣的话题,因为动态衍射通常发生在诸如Si晶体之类的完美晶体中。然而,据我们所知,尚无关于蛋白质晶体显示出清晰的动态衍射的报道。我们想知道,与高质量的Si晶体相比,蛋白质晶体的完善性是否仍然较低。在这里,我们目前观察到蛋白质晶体(例如葡萄糖异构酶晶体)的摇摆曲线的振荡曲线。振荡轮廓与衍射动力学理论所预测的轮廓吻合良好。我们证明了即使在蛋白质晶体中也会发生动态衍射。

更新日期:2018-04-04
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