A new automated chemiluminescence method resorting to sequential injection analysis (SIA) was developed to rapidly determine biochemical oxygen demand (BOD). The assay is based on the redox reaction between quinone and Baker’s yeast in the presence of organic substances. The formed active oxygen species reacted with luminol, under the catalytic action of ferricyanide, and increased chemiluminescence signal. The automation of the assay ensured a precise control of the reaction conditions and enabled a reduction of more than 75-times in the reagents consumption and effluents production comparatively to BOD₅. The sampling rate was widely improved with a flow rate of 8 cycles per hour. The method was applied to determine the BOD of ionic liquids (ILs) incorporating different chemical elements and deep eutectic solvents (DESs) combining choline chloride with varying hydrogen-bond donors. Differences in BOD and biodegradability were observed between tested compounds, with DESs showing, in general, higher BOD values and greater biodegradation than ILs. The results obtained in the developed bioassay demonstrated statistical correlation with the BOD₅ method. Therefore, the developed methodology is a simple, economic and high-throughput alternative screening bioassay to the conventional method, with the potential to preliminarily assess the potential biodegradability of chemicals in the environment.

中文翻译：

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啤酒酵母微流化学发光系统用于快速生化需氧量的测量

开发了一种依靠顺序注射分析（SIA）的新的自动化学发光方法来快速确定生化需氧量（BOD）。该测定基于在有机物质存在下醌和贝克酵母之间的氧化还原反应。形成的活性氧在铁氰化物的催化作用下与鲁米诺反应，并增加化学发光信号。测定的自动化确保了对反应条件的精确控制，与BOD ₅相比，可减少75倍以上的试剂消耗和废水产生。每小时8个循环的流速大大提高了采样率。该方法用于测定结合了不同化学元素和深共熔溶剂（DES）的离子液体（ILs）的BOD，该溶液将氯化胆碱与不同的氢键供体结合在一起。在测试的化合物之间观察到BOD和生物降解能力的差异，与IL相比，DES总体上具有更高的BOD值和更大的生物降解性。在开发的生物测定中获得的结果证明与BOD ₅方法具有统计相关性。因此，所开发的方法是常规方法的一种简单，经济，高通量的筛选生物测定方法，具有初步评估环境中化学物质潜在生物降解能力的潜力。