We present a pilot study on the effects of milk protein fractions [α_S1-casein (CN), α_S2-CN, κ-CN, β-CN, and a mix of α-lactalbumin (α-LA) and β-lactoglobulin (β-LG)] from different animal species (bovine, ovine, and caprine) on pro- and anti-inflammatory cytokines and oxidative status in cultured peripheral blood mononuclear cells from children with generalized epilepsy. Peripheral blood mononuclear cells (PBMC) were obtained by density gradient from blood of 10 children with generalized epilepsy (5 males; mean age 33.6 ± 5.4 mo) and 10 controls (5 males; mean age 35.6 ± 6.8 mo). Children with epilepsy were grouped according to cytokine levels as follows: children with epilepsy having low levels of cytokines not different from those of control children (LL-EC); children with epilepsy having cytokine levels at least 5-fold higher (medium levels) than those of control children (ML-EC); and children with epilepsy having cytokine levels at least 10-fold higher (high levels) than those of control children (HL-EC). The production of tumor necrosis factor-α (TNF-α), IL-10, IL-6, and IL-1β was studied in cultured PBMC incubated with α_S1-CN, α_S2-CN, κ-CN, β-CN, and a mix of α-LA and β-LG from bovine, caprine, and ovine milks. The levels of reactive oxygen and nitrogen species (ROS/RNS) and catalase activity were assessed in cultured supernatant. In the HL-EC group, β-CN from small ruminant species (ovine and caprine) induced the highest levels of TNF-α, whereas PBMC incubated with α_S2-CN from ovine milk and the mix of β-LG and α-LA from all tested milk species had the lowest levels of TNF-α. Within the HL-EC group, production of IL-1β was higher for bovine and ovine α_S2-CN fractions and lower for caprine and ovine β-CN and κ-CN. In the HL-EC group, IL-6 was higher in cultured PBMC incubated with α_S2-CN from bovine and ovine milk than from caprine milk. The cytokine IL-10 did not differ among milking species. The highest levels of ROS/RNS were found after incubation of PBMC with the β-CN fraction in bovine milk. Catalase activity was higher in PBMC cultured with β-CN isolated from bovine and caprine milk and with α_S1-CN from ovine milk.

我们目前的乳蛋白质级分的[α的作用的初步研究_S1酪蛋白（CN），α _S2-CN，κ-CN，β-CN，以及来自不同动物物种（牛，绵羊和山羊）的α-乳白蛋白（α-LA）和β-乳球蛋白（β-LG）的混合物广泛性癫痫患儿外周血单个核细胞中的炎症细胞因子和氧化状态。通过密度梯度法从10名患有全身性癫痫的儿童（5名男性；平均年龄33.6±5.4个月）和10名对照（5名男性；平均年龄35.6±6.8个月）的血液中通过密度梯度获得外周血单个核细胞（PBMC）。根据细胞因子水平将癫痫患儿分为以下几类：癫痫患儿的细胞因子水平与对照患儿的水平无差异（LL-EC）；癫痫儿童的细胞因子水平（中等水平）至少比对照儿童（ML-EC）高5倍；癫痫儿童的细胞因子水平（高水平）至少比对照儿童（HL-EC）高10倍。用α培养的PBMC研究了肿瘤坏死因子-α（TNF-α），IL-10，IL-6和IL-1β的产生。_S1 -CN，α _S2 -CN，κ-CN，β-CN，和α-LA和β-LG的来自牛，山羊，绵羊和乳的混合物。在培养的上清液中评估了活性氧和氮物种（ROS / RNS）和过氧化氢酶活性的水平。在HL-EC基，β-CN从诱导的TNF-α的最高水平，而PBMC与α孵育小反刍动物（绵羊和山羊）_S2 -CN从绵羊乳和β-LG的混合和α-LA所有测试的牛奶种类中的TNF-α含量最低。内的HL-EC组，生产IL-1β的是牛和羊α更高_S2 -CN馏分并降低山羊和绵羊β-CN和κ-CN。在HL-EC组，IL-6是与α孵育培养的PBMC更高_S2牛乳和羊乳中的-CN比山羊乳中的-CN多。细胞因子IL-10在挤奶物种之间没有差异。将PBMC与牛乳中的β-CN组分孵育后，发现最高水平的ROS / RNS。用从牛和山羊奶中分离的β-CN和从羊乳中分离的_αS1 -CN培养的PBMC中过氧化氢酶活性更高。