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Multiwalled carbon nanotube modified microfluidic-based biosensor chip for nucleic acid detection
Sensors and Actuators B: Chemical ( IF 8.4 ) Pub Date : 2018-03-21 , DOI: 10.1016/j.snb.2018.03.118
Aditya Sharma Ghrera , Chandra Mouli Pandey , Bansi Dhar Malhotra

An impedimetric microfluidic–based biosensor was fabricated and investigated for quantification of the DNA sequence specific to chronic myelogenous leukemia (CML). The sensor chip was constructed by electrophoretic deposition of carboxyl-modified multiwalled carbon nanotubes (MWCNT) on the patterned (via wet chemical etching method) indium–tin–oxide (ITO) coated glass substrate. The MWCNT surface was immobilized with CML specific deoxyribonucleic acid probe, followed by sealing of the biochip with poly (dimethylsiloxane) microchannel for fluid control. This integrated miniaturized system was used to monitor complementary target DNA concentration by measuring the interfacial charge transfer resistance via hybridization. The presence of complementary DNA in buffer solution resulted in significant decrease in electrical conductivity of the interface thereby presenting a barrier for transport of the redox probe ions. Under optimal conditions, this microfluidic biochip exhibited good calibration range from 1fM to 1 μM and a response time of 60 s.



中文翻译:

用于核酸检测的多壁碳纳米管修饰微流体生物传感器芯片

制备了一种基于阻抗的微流控生物传感器,并进行了定量分析,该定量分析了慢性粒细胞性白血病(CML)特异的DNA序列。传感器芯片是通过将羧基修饰的多壁碳纳米管(MWCNT)电泳沉积在经过图案化(通过湿法化学蚀刻法)的氧化铟锡(ITO)的玻璃基板上而制成的。用CML特定的脱氧核糖核酸探针固定MWCNT表面,然后用聚二甲基硅氧烷微通道密封生物芯片以控制流体。该集成的小型化系统用于通过测量杂交的界面电荷转移阻力来监测互补靶DNA的浓度。缓冲溶液中互补DNA的存在导致界面电导率显着降低,从而为氧化还原探针离子的运输提供了障碍。在最佳条件下,这种微流体生物芯片表现出良好的校准范围,从1fM到1μM,响应时间为60 s。

更新日期:2018-03-21
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