Rapid detection and estimation of Lead (Pb²⁺) has been accomplished in randomly selected lipstick samples by a simple FRET-based chemosensor RNPC (Rhodamine-Naphthalimide Conjugate) through “turn on” fluorescence. Fluorescence intensities were compared with the fluorescence plot of RNPC-Pb²⁺ complex to evaluate the concentration of Pb²⁺ present in the sample solutions. NMR titrations and TD-DFT calculations were achieved in order to demonstrate the sensing mechanism and the electronic properties of the RNPC-Pb²⁺ complex. Different cell lines have been cultured for live cell imaging study to understand the in vitro sensing mechanism and to satisfy the biocompatibility of the probe RNPC.

通过简单的基于FRET的化学传感器RNPC（若丹明-萘二甲酰亚胺共轭物），通过“开启”荧光，可以对随机选择的口红样品中的铅（Pb ²⁺）进行快速检测和估计。将荧光强度与RNPC -Pb ²⁺复合物的荧光图进行比较，以评估样品溶液中Pb ²⁺的浓度。为了证明RNPC -Pb ^2+配合物的传感机理和电子性质，进行了NMR滴定和TD-DFT计算。培养了不同的细胞系用于活细胞成像研究，以了解体外传感机制并满足RNPC探针的生物相容性。