Liquid chromatography tandem mass spectrometry (LC-MS/MS) assays are considered the reference standard for serum steroid hormone analyses, while full urinary steroid profiles are only achievable by gas chromatography (GC–MS). Both LC-MS/MS and GC–MS have well documented strengths and limitations. Recently, commercial ultra-high performance supercritical fluid chromatography–tandem mass spectrometry (UHPSFC-MS/MS) systems have been developed. These systems combine the resolution of GC with the high-throughput capabilities of UHPLC. Uptake of this new technology into research and clinical labs has been slow, possibly due to the perceived increase in complexity. Here we therefore present fundamental principles of UHPSFC-MS/MS and the likely applications for this technology in the clinical research setting, while commenting on potential hurdles based on our experience to date.

液相色谱串联质谱分析法（LC-MS / MS）被认为是血清类固醇激素分析的参考标准，而完整的尿类固醇激素谱只能通过气相色谱法（GC-MS）实现。LC-MS / MS和GC-MS都有充分记录的优点和局限性。最近，已经开发出了商用超高性能超临界流体色谱-串联质谱（UHPSFC-MS / MS）系统。这些系统将GC的分辨率与UHPLC的高通量功能结合在一起。这项新技术在研究和临床实验室中的采用速度一直很慢，这可能是由于人们认为复杂性有所增加。因此，在这里，我们介绍了UHPSFC-MS / MS的基本原理以及该技术在临床研究环境中的可能应用，