Polycyclic aromatic hydrocarbons (PAHs) are ubiquitously found in the environment and have been proved to be prospectively associated with the risk of cancer. In this study, a simple method based on pipette-tip solid phase extraction (PT-SPE) and gas chromatography-mass spectrometry (GC–MS) has been firstly developed for the determination of 16 PAHs in human whole blood. Three-dimensional ionic liquid-ferrite functionalized graphene oxide nanocomposite (3D-IL-Fe₃O₄-GO) was used as sorbent in PT-SPE. Compared with conventional SPE method, the PT-SPE method was solvent-saving (1.0 mL), reusable (at least 10 times) and required less blood sample (200 μL). Affecting parameters on extraction efficiency were investigated and optimized. Under the optimized conditions, a good linearity was obtained and the recoveries of 16 PAHs at three spiked levels ranged from 85.0% to 115%. The limits of quantification (LOQs) were in the range of 0.007-0.013 μg/L. Furthermore, the developed method was successfully applied to the analysis of 16 PAHs in 14 human blood samples. The results showed that the predominant PAHs in human whole blood was low-molecular-weight PAHs, with the rank order phenanthrene (PHE)> naphthalene (NAP)> fluorene (FLU)> fluoranthene (FLT)> pyrene (PYR). Because of its simplicity, accuracy and reliability, the PT-SPE method combined with GC–MS demonstrated the applicability for clinical analysis and provided more information for PAHs exposure studies.

多环芳烃（PAHs）在环境中无处不在，并且已被证明与癌症风险有潜在联系。在这项研究中，首先开发了一种基于移液器固相萃取（PT-SPE）和气相色谱-质谱（GC-MS）的简单方法，用于测定人全血中的16种PAHs。三维离子液体铁氧体功能化氧化石墨烯纳米复合材料（3D-IL-Fe ₃ O ₄-GO）用作PT-SPE中的吸附剂。与传统的SPE方法相比，PT-SPE方法节省了溶剂（1.0 mL），可重复使用（至少10次），所需的血样更少（200μL）。研究并优化了影响提取效率的参数。在优化的条件下，获得了良好的线性，三个加标水平下的16种PAHs的回收率在85.0％至115％之间。定量限（LOQ）在0.007-0.013μg/ L的范围内。此外，所开发的方法已成功地用于分析14个人血样品中的16 PAHs。结果表明，人全血中主要的PAHs为低分子量PAHs，其排位顺序为菲（PHE）>萘（NAP）>芴（FLU）>荧蒽（FLT）>（（PYR）。由于其简单性，