Allergenic disperse dyes are a group of environmental contaminants, which are toxic and mutagenic to human beings. In this work, a method of dispersive solid-phase extraction (d-SPE) using graphene-coated polystyrene-divinylbenzene ([email protected]) microspheres coupled with supercritical fluid chromatography (SFC) was proposed for the rapid determination of 10 allergenic disperse dyes in industrial wastewater samples. [email protected] microspheres were synthesized by coating graphene (G) sheets onto polystyrene-divinylbenzene (PS-DVB) polymers. Such novel sorbents were employed in d-SPE for the purification and concentration of allergenic disperse dyes in wastewater samples prior to the determination by SFC with UV detection. To achieve the maximum extraction efficiency for the target dyes, several parameters influencing d-SPE process such as sorbent dosage, extraction time, desorption conditions were investigated. SFC conditions including stationary phase, modifier composition and percentage, column temperature, backpressure and flow rate were optimized to well separate the allergenic disperse dyes. Under the optimum conditions, satisfactory linear relationship (R ≥ 0.9989) was observed with the concentration of dyes ranging from 0.02 to 10.0 μg/mL. The limits of detection (LOD, S/N = 3) for the ten dyes were in the range of 1.1–15.6 ng/mL. Recoveries for the spiked samples were between 89.1% and 99.7% with relative standard deviations (RSD) lower than 10.5% in all cases. The proposed method is time-saving, green, precise and repeatable for the analysis of the target dyes. Furthermore, the application of [email protected] based d-SPE process can be potentially expanded to isolate and concentrate other aromatic compounds in various matrices and supercritical fluid chromatography methodology featuring rapidity, accuracy and green will be an ideal candidate for the analysis of these compounds.

变应原性分散染料是一组环境污染物，对人类有毒和致突变。在这项工作中，提出了一种使用石墨烯包覆的聚苯乙烯-二乙烯基苯（[电子邮件保护]）微球和超临界流体色谱法（SFC）进行分散固相萃取（d-SPE）的方法，用于快速测定10种致敏分散染料。在工业废水样品中。通过将石墨烯（G）片材涂覆到聚苯乙烯-二乙烯基苯（PS-DVB）聚合物上来合成[受电子邮件保护的]微球。此类新型吸附剂已在d-SPE中用于纯化和浓缩废水样品中的致敏分散染料，然后通过SFC和UV检测进行测定。为了实现目标染料的最大提取效率，研究了影响d-SPE工艺的几个参数，如吸附剂量，萃取时间，解吸条件。优化了SFC条件，包括固定相，改性剂组成和百分比，柱温，背压和流速，以很好地分离致敏分散染料。在最佳条件下，染料浓度为0.02至10.0μg/ mL时，观察到令人满意的线性关系（R≥0.9989）。十种染料的检出限（LOD，S / N = 3）在1.1-15.6 ng / mL范围内。在所有情况下，加标样品的回收率在89.1％至99.7％之间，相对标准偏差（RSD）低于10.5％。所提出的方法省时，绿色，精确且可重复，可用于目标染料的分析。此外，