Hydrogen peroxide (H₂O₂) is an oxidative stress marker in various disease. The local concentration of H₂O₂ is related to cancer, a reliable and simple assay detecting H₂O₂ level in cells is therefore critical. Here, we have developed a fluorescence probe PEP-Npb1-Cy3 which can be used to detect H₂O₂ in living ovarian cancer cells. The probe is developed based on employing the oxide reactivity of 1, 8-naphthalimide boric acid towards H₂O₂, which yields a turn-on fluorescence signal and exhibits high selectivity and sensitivity. Our collective results support the applicability of PEP-Npb1-Cy3 as a powerful tool for imaging both exogenous and endogenous H₂O₂-involving biological events in ovarian cancer cells, which may have further biomedical applications.

过氧化氢（H ₂ O ₂）是各种疾病中的氧化应激标记。H ₂ O ₂的局部浓度与癌症有关，因此可靠，简单的检测细胞中H ₂ O ₂水平的方法至关重要。在这里，我们开发了一种荧光探针PEP-Npb1-Cy3，可用于检测卵巢癌细胞中的H ₂ O ₂。该探针是基于利用1，8，萘二甲酰亚胺硼酸对H ₂ O ₂的氧化物反应性而开发的产生一个开启的荧光信号，并具有很高的选择性和灵敏度。我们的集体结果支持PEP-Npb1-Cy3作为对卵巢癌细胞中涉及外源性和内源性H ₂ O _2的生物学事件进行成像的有力工具，这可能具有进一步的生物医学应用。