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Photochemical Barcodes
Journal of the American Chemical Society ( IF 15.0 ) Pub Date : 2018-03-21 , DOI: 10.1021/jacs.8b00887
Sicheng Tang 1 , Yang Zhang 1 , Pravat Dhakal 1 , Laura Ravelo 1 , Cheyenne L. Anderson 1 , Kevin M. Collins 1 , Françisco M. Raymo 1
Affiliation  

A photochemical strategy to encode fluorescence signals in vivo with spatial control was designed around the unique properties of a photoactivatable borondipyrromethene (BODIPY). The photoinduced disconnection of two oxazines, flanking a single BODIPY, in two consecutive steps produces a mixture of three emissive molecules with resolved fluorescence inside polymer beads. The relative amounts and emission intensities of the three fluorophores can be regulated precisely in each bead by adjusting the dose of activating photons to mark individual particles with distinct codes of fluorescence signals. The visible wavelengths and mild illumination sufficient to induce these transformations permit the photochemical barcoding of beads also in living nematodes. Different regions of the same animal can be labeled with distinct barcodes to allow the monitoring of their dynamics for long times with no toxic effects. Thus, our photochemical strategy for the generation of fluorescence barcodes can produce multiple and distinguishable labels in the same biological sample to enable the spatiotemporal tracking of, otherwise indistinguishable, targets.

中文翻译:

光化学条码

围绕可光活化的硼二吡咯亚甲基 (BODIPY) 的独特特性设计了一种在体内编码具有空间控制的荧光信号的光化学策略。两个恶嗪在单个 BODIPY 侧翼的光诱导断开,在两个连续步骤中产生三个发射分子的混合物,聚合物珠内具有解析的荧光。通过调整激活光子的剂量以标记具有不同荧光信号代码的单个粒子,可以精确调节每个珠子中三种荧光团的相对数量和发射强度。足以诱导这些转变的可见波长和温和的光照允许在活的线虫中对珠子进行光化学条形码编码。同一动物的不同区域可以用不同的条形码标记,以便长时间监测它们的动态,而不会产生毒性作用。因此,我们用于生成荧光条码的光化学策略可以在同一生物样本中产生多个可区分的标签,以实现对无法区分的目标的时空跟踪。
更新日期:2018-03-21
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