A blood cross-matching test should be carried out to prevent a hemolytic transfusion reaction as the final verification step. To simplify complicated procedures of a conventional blood cross-matching test requiring bulky systems and skilled people, we present a finger-actuated microfluidic device for the blood cross-matching test. Although finger actuation is a simple action that anyone can easily accomplish, there would be a variation in the individual finger actuation that may induce the user-dependent errors of the device. Therefore, the working principle of the finger-actuated microfluidic device is newly designed to reduce the user-dependent errors by indirectly controlling the pressure of fluidic channels. The constant volume was repeatedly dispensed by pushing and releasing a pressure chamber regardless of the different pushed depths of the pressure chamber, the pushing time interval, and the end-users. The dispensed volume was linearly increased according to the number of pushing times applied to the pressure chamber and determined by adjusting the diameter of an actuation chamber. In addition, multiple fluids can be dispensed with a desirable ratio by pushing and releasing the pressure chamber. Finally, a finger-actuated microfluidic device for the blood cross-matching test was developed, which can simultaneously actuate four fluidic channels. After loading 50 μL of whole blood samples from a donor and a recipient into two inlets of the device, the blood plasma from each individual was separated through the two plasma separation membranes. The blood cross-matching test results can be achieved by cross-reacting the donor's blood plasma with the recipient's whole blood as well as the donor's whole blood with the recipient's blood plasma by pushing and releasing only a single pressure chamber within 10 min.

中文翻译：

---

手指驱动的微流控设备用于血液交叉匹配测试†

作为最终验证步骤，应进行血液交叉匹配测试以防止溶血性输血反应。为了简化需要庞大系统和熟练技术人员的常规血液交叉匹配测试的复杂程序，我们提出了一种用于血液交叉匹配测试的手指驱动微流控设备。尽管手指致动是任何人都可以轻松完成的简单动作，但是各个手指致动会产生变化，这可能会导致设备的用户相关错误。因此，手指驱动的微流体装置的工作原理是新设计的，以通过间接控制流体通道的压力来减少与用户有关的误差。不管压力室的推动深度，推动时间间隔和最终用户如何，都可以通过推动和释放压力室来重复分配恒定体积。分配的体积根据施加到压力室的推动次数线性增加，并通过调节致动室的直径来确定。另外，可以通过推动和释放压力室以期望的比例分配多种流体。最后，开发了一种用于血液交叉匹配测试的手指驱动微流体装置，该装置可以同时驱动四个流体通道。将来自供体和受体的50μL全血样品装入设备的两个入口后，通过两个血浆分离膜分离每个个体的血浆。