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Stress-related genes promote Edwardsiella ictaluri pathogenesis
PLOS ONE ( IF 3.7 ) Pub Date : 2018-03-19 , DOI: 10.1371/journal.pone.0194669
Ali Akgul , Ayfer Akgul , Mark L. Lawrence , Attila Karsi

Edwardsiella ictaluri is a Gram-negative facultative anaerobic rod and the causative agent of enteric septicemia of channel catfish (ESC), which is one of the most prevalent diseases of catfish, causing significant economic losses in the catfish industry. E. ictaluri is resistant to complement system and macrophage killing, which results in rapid systemic septicemia. However, mechanisms of E. ictaluri stress responses under conditions of host environment are not studied well. Therefore, in this work, we report E. ictaluri stress responses during hydrogen peroxide, low pH, and catfish serum stresses as well as during catfish invasion. E. ictaluri stress responses were characterized by identifying expression of 13 universal stress protein (USP) genes (usp01-usp13) and seven USP-interacting protein genes (groEL, groES, dnaK, grpE, and clpB, grpE, relA). Data indicated that three usp genes (usp05, usp07, and usp13) were highly expressed in all stress conditions. Similarly, E. ictaluri heat shock proteins groEL, groES, dnaK, grpE, and clpB were highly expressed in oxidative stress. Also, E. ictaluri grpE and relA were highly expressed in catfish spleen and head kidney. These findings contribute to our understanding of stress response mechanisms in E. ictaluri stress response, and stress-related proteins that are essential for E. ictaluri could be potential targets for live attenuated vaccine development against ESC.



中文翻译:

与压力相关的基因促进了爱德华氏菌的发病机理

Edwardsiella ictaluri是革兰氏阴性兼性厌氧菌,是channel鱼肠道败血症的病原体,它是cat鱼最普遍的疾病之一,在the鱼行业造成了巨大的经济损失。Ëictaluri对补体系统和巨噬细胞杀伤具有抵抗力,可导致快速的系统性败血症。但是,E的机制。对宿主环境下的ictaluri应激反应的研究还不够好。因此,在这项工作中,我们报告ē。在过氧化氢,低pH和cat鱼血清应激以及cat鱼入侵期间的ictaluri应激反应。Ë通过鉴定13种通用应激蛋白(USP)基因(usp01 - usp13)和7与USP相互作用的蛋白基因(groELgroESdnaKgrpEclpBgrpErelA)的表达来表征ictaluri应激反应。数据表明,三个usp基因(usp05usp07usp13)在所有胁迫条件下均高表达。同样,Eitaltal热休克蛋白groELgroESdnaKgrpEclpB在氧化应激中高表达。另外,Eitaltal grpErelAcat鱼脾脏和头部肾脏中高表达。这些发现有助于我们理解E中的应激反应机制。ictaluri应激反应以及E必需的应激相关蛋白。ictaluri可能是针对ESC的减毒活疫苗开发的潜在目标。

更新日期:2018-03-20
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