We have previously characterized Pir32, a Candia albicans cell wall protein that we found to be involved in filamentation, virulence, chitin deposition, and resistance to oxidative stress. Other than defining the cell shape, the cell wall is critical for the interaction with the surrounding environment and the point of contact and interaction with the host surface. In this study, we applied tandem mass spectrometry combined with bioinformatics to investigate cell wall proteome changes in a pir32 null strain. A total of 16 and 25 proteins were identified exclusively in the null mutant strains grown under non-filamentous and filamentous conditions. These proteins included members of the PGA family with various functions, lipase and the protease involved in virulence, superoxide dismutases required for resisting oxidative stress, alongside proteins required for cell wall remodeling and synthesis such as Ssr1, Xog1, Dfg5 and Dcw1. In addition proteins needed for filamentation like Cdc42, Ssu81 and Ucf1, and other virulence proteins such as Als3, Rbt5, and Csa2 were also detected. The detection of these proteins in the mutant and their lack of detection in the wild type can explain the differential phenotypes previously observed.

我们先前已经鉴定了Pir32，一种白色念珠菌细胞壁蛋白，我们发现它与丝化，毒力，几丁质沉积和抗氧化应激有关。除了定义细胞的形状外，细胞壁对于与周围环境的相互作用以及与宿主表面的接触和相互作用点至关重要。在这项研究中，我们应用串联质谱结合生物信息学研究了pir32中细胞壁蛋白质组的变化。空应变。在非丝状和丝状条件下生长的无效突变菌株中，仅鉴定出总共16和25种蛋白质。这些蛋白质包括具有各种功能的PGA家族成员，参与毒性的脂肪酶和蛋白酶，抗氧化应激所需的超氧化物歧化酶，以及细胞壁重塑和合成所需的蛋白质，例如Ssr1，Xog1，Dfg5和Dcw1。此外，还检测到了细丝化所需的蛋白质，例如Cdc42，Ssu81和Ucf1，以及其他毒力蛋白质，例如Als3，Rbt5和Csa2。在突变体中检测到这些蛋白质，而在野生型中却没有检测到，可以解释先前观察到的差异表型。