For the first time, we are reporting a novel type of dual fluorescence temperature-sensitive DNA-templated silver nanocluster (AgNC) pair, which contains two pieces of single-stranded AgNC in proximity through hybridization. Both the chameleon AgNC pairs, A-NCP and B-NCP, possess two bright fluorescence peaks that achieve sensitive variations corresponding to temperature change from 15 to 45 °C. With the increase in temperature, one of the fluorescence emissions of A-NCP (A-FL₅₇₀) increases, while the other (A-FL₆₄₀) decreases. However, both the emissions of B-NCP (B-FL₆₈₅ and B-FL₆₂₀) decrease simultaneously. Therefore, A-NCP shows a remarkable fluorescence color variation from orange to yellow, while the fluorescence color of B-NCP changes from orange to colorless, with increase in temperature. Moreover, the temperature responding linear range of A-NCP can be regulated by adjusting the structures and sequences of assistant DNA templates. It is assumed that the two single-stranded segmental AgNCs are integrated together as they are assembled into AgNC pairs, leading to a dramatic variation in fluorescence properties. The temperature-sensitive phenomenon is due to the dehybridization-induced separation of two pieces of segmental AgNC, caused by temperature increase. The temperature-sensitive AgNC pairs have been successful in indicating the temperature of living cells, showing the potential for a new application of silver nanocluster as a nanothermometer with adjustable response range, bringing novel insight into the regulatory mechanism of AgNC fluorescence variation.

中文翻译：

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用于细胞内测温的新型双荧光温度敏感变色龙DNA模板银纳米簇对

首次，我们报道了一种新型的双荧光温度敏感DNA模板银纳米簇（AgNC）对，其中包含通过杂交在邻近的两片单链AgNC。变色龙AgNC对（A-NCP和B-NCP）都具有两个明亮的荧光峰，这些峰实现了从15到45°C的温度变化所对应的敏感变化。随着温度的升高，A-NCP的一种荧光发射（A-FL ₅₇₀）增加，而另一种（A-FL ₆₄₀）减少。但是，B-NCP的排放（B-FL ₆₈₅和B-FL ₆₂₀）同时减少。因此，随着温度的升高，A-NCP的荧光颜色从橙色变为黄色，而B-NCP的荧光颜色从橙色变为无色。此外，可以通过调节辅助DNA模板的结构和序列来调节A-NCP的温度响应线性范围。假定两个单链节段AgNC在组装成AgNC对时会整合在一起，从而导致荧光性质发生巨大变化。温度敏感现象是由于温度升高引起的两段片段AgNC的脱杂化分离所致。对温度敏感的AgNC对已经成功地表明了活细胞的温度，