A fluorescence probe based on cinnamyl pyrazoline was developed and synthesized. The probe detected Zn²⁺ ion that was mixed with a series of cations in aqueous ethanol with high selectivity and high sensitivity. After adding Zn²⁺, the fluorescence intensity was significantly enhanced, with a 56-fold increase. The detection limits and binding constants of 2.95 × 10⁻⁸ M and 1.468 × 10⁴ M⁻¹ were calculated by a fluorescence titration experiment. The 1 : 1 binding chemometrics of the 1–Zn²⁺ complex was analyzed by Job's plot. The probe showed good reversibility when adding PPi. Additionally, the “turn-on” fluorescent probe with excellent membrane permeability was satisfactorily applied to microscopic imaging for the detection of Zn²⁺ in baby hamster kidney (BHK)-21 cells.

中文翻译：

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基于肉桂基吡唑啉衍生物的锌离子高度选择性“开启”荧光传感器及其在活细胞中的成像†

开发并合成了基于肉桂基吡唑啉的荧光探针。该探针以高选择性和高灵敏度检测到在乙醇水溶液中与一系列阳离子混合的Zn ²⁺离子。添加Zn ^2+后，荧光强度显着增强，增加了56倍。通过荧光滴定实验计算出2.95×10 ^-8 M和1.468×10 ⁴ M ^-1的检测限和结合常数。1 –Zn ²⁺的1：1结合化学计量学乔布斯的情节分析了复杂的事物。添加PPi时，探针显示出良好的可逆性。此外，具有出色的膜渗透性的“开启”荧光探针已令人满意地应用于显微成像，以检测小仓鼠肾脏（BHK）-21细胞中的Zn ²⁺。